基于响应面法和UPLC-MS/MS优化舒心安神膏的提取工艺  

Optimization of extraction process of Shuxin Anshen ointment based on response surface method and UPLC-MS/MS

作　　者：代婷媛 徐福平[3] 王剑波 赵军宁[2] 杨志敏[3] 尤晓舟 彭一淼 郭延垒 王云红[4] DAI Tingyuan;XU Fuping;WANG Jianbo;ZHAO Junning;YANG Zhimin;YOU Xiaozhou;PENG Yimiao;GUO Yanlei;WANG Yunhong(Department of Pharmacology,Shaanxi University of Chinese Medicine,Key Laboratory of PharmacoDynamics and Substance Basis of Traditional Chinese Medicine,Shaanxi Provincial Administration of Traditional Chinese Medicine,Engineering Research Center of Traditional Chinese Medicine Brain Health Industry,Xi′an 712046,China;Sichuan Academy of Chinese Medicine Sciences,Translational Chinese Medicine Key Laboratory of Sichuan Province;Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Provincial Hospital of Traditional Chinese Medicine;Pharmacology Laboratory of Traditional Chinese Medicine,Chongqing Academy of Traditional Chinese Materia Medica)

机构地区：[1]陕西中医药大学药理学教研室,陕西省中医药管理局中药药效机制与物质基础重点研究室,中医药脑健康产业陕西省高校工程研究中心,西安712046 [2]四川省中医药科学院,中医药转化医学四川省重点实验室 [3]广州中医药大学第二附属医院,广东省中医院 [4]重庆市中药研究院中药药理研究室

出　　处：《山西医科大学学报》2024年第3期354-361,共8页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81974560);广东省基础与应用基础研究企业联合基金资助项目(2022A1515220073);广东省中医院颜德馨学术经验传承工作室建设项目(中医二院[2014]89号-6)。

摘　　要：目的利用响应面法优化舒心安神膏的提取工艺。方法以超高效液相色谱法同时测定的莫诺苷、马钱苷、芍药苷、斯皮诺素、6-姜辣素含量,出膏率及总多糖含量为综合评分指标,分别考察加水倍数、提取时间、提取次数对舒心安神膏提取工艺的影响。在单因素试验基础上,采用响应面法优化舒心安神膏的提取工艺。结果经过单因素试验及响应面法优化后的最佳工艺条件为:加10倍水,煎煮2次,每次提取90 min。此条件下综合评分预测值与实测值相比,RSD值为0.82%。结论通过响应面法优化得到的舒心安神膏提取工艺合理、可行,可为其后续的开发研究提供实验参考。Objective To optimize the extraction process of Shuxin Anshen ointment by response surface method.Methods With the contents of monoxide,maggianin,paeoniflorin,spinoside,6-gingerol simultaneously determined by ultra-high performance liquid chromatography,the extraction rate,and the total polysaccharide content as the comprehensive score indexes,the effects of water adding ratio,extraction time,and extraction times on the extraction process of Shuxin Anshen ointment were investigated by a single-factor test.Based on the single factor results,the extraction process of Shuxin Anshen ointment was optimized by response surface method.Results After single factor test and response surface method optimization,the optimal process conditions were as follows:adding 10 times water,extracting twice,and extracting 90 min each time.Under this condition,the RSD of the predicted comprehensive score and the measured score was 0.82%.Conclusion The extraction process of Shuxin Anshen ointment optimized by the response surface method is reasonable and feasible,which can provide an experimental reference for its subsequent development and research.

关 键 词：UPLC-MS/MS 响应面法 舒心安神膏 熵权法 

分 类 号：TQ460.6[化学工程—制药化工]