款冬花不同炮制品中生物碱的肝脾毒性研究  

Study on hepatic and spleen toxicity of alkaloids in different processed products of Farfare flos

作　　者：赖岳晓 刘佩沂 田素英[2] 梁晓滢 LAI Yue-xiao;LIU Pei-yi;TIAN Su-ying(Huangpu People's Hospital in Zhongshan City,Zhongshan 528400,China.;不详)

机构地区：[1]中山市黄圃人民医院,广东中山528400 [2]广东药科大学,广州510006

出　　处：《中国处方药》2024年第4期25-30,共6页Journal of China Prescription Drug

基　　金：中山市科技局科研项目(2017B1088)。

摘　　要：目的对款冬花不同炮制品中生物碱的肝脾毒性进行初步研究以获得最佳炮制方法。方法分别给昆明种小鼠灌胃款冬花生品、蜜炙品、甘草水制品、甘草水制蜜炙共制品以及用甘草水炼蜜的蜜炙品所提取的总生物碱,连续给药8 d后,测定体重变化,计算肝脏指数,测定血清中白蛋白(ALB)、总胆汁酸(TBA)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)含量,对小鼠的肝脏、脾脏进行病理组织学检查。结果与空白组比较,生品组肝脏指数升高明显;各炮制品组与生品组相比,各炮制品组小鼠体重均有小幅度的增加,肝脏指数也有降低。与空白组比较,生品组的白蛋白、总胆汁酸、AST水平明显升高;各炮制品组与生品组相比都有所下降。病理切片显示,小鼠的脾脏未见明显病变,肝脏切片中生品组和各炮制品组的肝细胞都出现不同程度的融合性坏死,可见肝细胞局灶性,水性变样以及汇管区炎症,但甘草水制组和甘草水制蜜炙组坏死融合程度较为轻微。结论不同炮制品的款冬花生物碱含量对小鼠肝脏毒性存在差异,其中以甘草水制和甘草水制蜜炙共制款冬花对小鼠肝脏毒性较小,减毒效果较好。Objective To preliminary study hepatic and spleen toxicity of alkaloids in different processed products of Farfare flos.Methods Kunming mice were given the alkaloid in intragastric administration of Farfare flos from raw product,honeyfried processed product,liquorice processed product,liquorice and honeyfried co-production processed product,refined hone by licorice water processed product for successive 8 day.Then measuring body weight changes,calculating levels of liver index,measure the albumin(ALB),total bile acid(TBA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and taking the liver and spleen of the mice for histopathological examination.Results Compared with the blank group,the liver index of the raw product group increased significantly;compared with the raw product group,mice in each processed product group showed a slight increase in body weight and a decrease in liver index.Compared with the blank group,the levels of albumin,total bile acid,and AST in the raw product group were significantly increased;compared to the raw product group,there has been a decrease in each processed product group.Pathological sections showed no significant lesions in the spleen of the mice.In the liver sections,liver cells from the raw and processed product groups showed varying degrees of fusion necrosis,indicating focal and watery changes in liver cells,as well as inflammation in the portal area.However,the degree of necrosis and fusion was relatively mild in the liquorice processing,liquorice and honeyfried co-production processing groups.Conclusion There are differences in the alkaloid content of different processed products of Farfare flos on the toxicity of mouse liver.Among them,liquorice processed,liquorice and honeyfried co-production processed Farfare flos products have lower toxicity to mouse liver and better detoxification effect.

关 键 词：款冬花炮制品 生物碱 肝脏毒性 血清生化指标 病理组织学 

分 类 号：R283[医药卫生—中药学]