乳香提取物调控Wnt/β-catenin信号通路抑制结直肠癌细胞侵袭转移的作用机制研究  

Study on the Mechanism of Boswellia Extract in Regulating the Wnt/β-catenin Pathway to Inhibit Invasion and Metastasis of Colorectal Cancer Cells

作　　者：邓兰 杨嘉欣 李志明 吴辉渊[1] DENG Lan;YANG Jiaxin;LI Zhiming;WU Huiyuan(Department of Oncology,Affiliated Hospital of Jiangxi University of Chinese Medicine,Jiangxi Province,Nanchang 330000,China)

机构地区：[1]江西中医药大学附属医院肿瘤科,江西南昌330000

出　　处：《中国中医药现代远程教育》2024年第11期156-159,共4页Chinese Medicine Modern Distance Education of China

基　　金：江西省卫生健康委员会一般项目【No.202310700】。

摘　　要：目的研究乳香提取物对人结肠癌细胞HCT-116增殖和侵袭转移的影响及可能的作用机制。方法体外培养人结肠癌细胞珠HCT-116,使用不同浓度乳香提取物乙酰基-11-酮基-β-乳香酸(AKBA)分别作用于细胞相同时间后,以CCK-8实验、Transwell细胞侵袭实验、划痕实验检测其对细胞增殖和侵袭转移的影响,以Western blotting、荧光定量PCR等方法探究其对细胞侵袭转移相关蛋白、mRNA表达的影响。结果AKBA可抑制人结肠癌细胞HCT-116的增殖、迁移能力,且呈剂量依赖(P<0.05);AKBA可抑制C-Myc、β-catenin的蛋白和mRNA表达,且呈剂量依赖(P<0.05)。结论AKBA可以抑制人结肠癌细胞HCT-116的增殖和迁移,诱导细胞凋亡。这可能与其调控Wnt/β-catenin信号通路相关。Objective To investigate the effects of Boswellia serrata extract(AKBA)on the proliferation and invasion and metastasis of human colon cancer cell line HCT-116 and its possible mechanism of action.Methods Human colon cancer cell beads HCT-116 were cultured in vitro and treated with different concentrations of mastic extract acetyl-11-keto-β-mastic acid(AKBA)for the same time.The effects of AKBA on cell proliferation,invasion and metastasis were detected by CCK-8 assay,Transwell cell invasion assay and scratch assay blotting and fluorescence quantitative PCR were used to investigate the effects on the expression of proteins and mRNA related to cell invasion and metastasis.Results AKBA inhibited the proliferation and migration ability of cells HCT-116 cells in a dose-dependent manner(P<0.05).AKBA inhibited the protein expression and mRNA expression of C-Myc andβ-catenin in a dose-dependent manner(P<0.05).Conclusion AKBA inhibited the proliferation and migration of human colon cancer cells HCT-116 and induced apoptosis,which may be related to the Wnt/β-catenin signalling pathway.

关 键 词：乳香 乙酰基-11-酮基-β-乳香酸 结直肠癌 侵袭转移 WNT/Β-CATENIN信号通路 实验研究 

分 类 号：R285[医药卫生—中药学]