6株布顿大麦内生真菌耐盐性研究  

Study on salt tolerance of six strains of endophytic fungi in Hordeum bogdanii

作　　者：滕天欣 张恩会 高军[3] 陈胜 陈水红[1] TENG Tian-xin;ZHANG En-hui;GAO Jun;CHEN Sheng;CHEN Shui-hong

机构地区：[1]塔里木大学生命科学与技术学院塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300 [2]烟台大学生命科学学院,山东烟台264005 [3]塔里木大学动物科学与技术学院,新疆阿拉尔843300

出　　处：《饲料研究》2024年第6期70-78,共9页Feed Research

基　　金：国家自然基金项目(项目编号:32260356、31660689);塔里木大学校长基金项目(项目编号:TDZKZD202201);塔里木大学研究生科研创新项目(项目编号:TDGRI202207)。

摘　　要：试验旨在明确不同盐浓度胁迫对大麦内生真菌的菌落形态等影响。对来自新疆不同地域布顿大麦中筛选到的形态不同的6株内生真菌菌株进行耐盐性试验,研究了菌株在氯化钠、硫酸钠不同盐浓度的PDA培养基上的生长情况。结果显示:供试菌株在氯化钠8个盐浓度(0、100、200、400、600、800、900、1000mmol/L)培养基和硫酸钠5个盐浓度(0、100、200、400、600mmol/L)的培养基中均能生长;同一盐浓度处理下,菌株在氯化钠盐培养基中的耐盐性整体高于硫酸钠盐。菌株A的内生真菌菌株在硫酸钠盐中生长极不耐受,出现菌丝褐化现象;菌株F的长势最差,菌株D的长势最好。在100mmol/L氯化钠处理下,菌株B的内生真菌菌落直径大于不加盐对照组。研究表明,试验结果有助于系统地了解布顿大麦内生真菌的耐盐性,为盐碱地区的土壤沙化治理选育植物提供参考。The purpose of this experiment was to clarify the effects of different salt concentrations on the colony morphology of endophytic fungi in Hordeum bogdanii.Six strains of endophytic fungi with different morphology were screened from Hordeum bogdanii from different regions of Xinjiang,and their growth on PDA medium with different salt concentrations of sodium chloride and sodium sulfate was studied.The results showed that the tested strains could grow in the medium with eight concentrations of sodium chloride(0,100,200,400,600,800,900,1000mmol/L)and five concentrations of sodium sulfate(0,100,200,400,600mmol/L).Under the same salt concentration treatment,the salt tolerance of the strain in sodium chloride medium was higher than that in sodium sulfate medium.The endophytic fungus strain of strain A was extremely intolerant in sodium sulfate salt,and its hyphae were browned.Strain F had the worst growth and strain D had the best growth.Under 100mmol/L sodium chloride treatment,the diameter of endophytic fungi colony of strain B was larger than that of the control group without salt.The study indicates that the results are helpful to understand systematically the salt tolerance of endophytic fungi in Hordeum bogdanii,and provide a reference for breeding plants for soil desertification control in saline-alkali areas.

关 键 词：布顿大麦 内生真菌 耐盐性 生理学特征 

分 类 号：S816[农业科学—饲料科学]