以卡托普利为模板银纳米团簇的制备及生物硫醇的检测研究  

Construction of Fluorescent Silver Nanoclusters Using Captopril as Template for Detecting the Biothiols

作　　者：王东新[1,2] 张慧慧 乔增杰 崔宏 WANG Dongxin;ZHANG Huihui;QIAO Zengjie;CUI Hong(Department of Public Health Laboratory Sciences,School of Public Health,Shanxi Medical University,Taiyuan 030001;Key Laboratory of Coal Environmental Pathogenicity and Prevention,Ministry of Education,Taiyuan,030001)

机构地区：[1]山西医科大学公共卫生学院卫生检验教研室,山西太原030001 [2]煤炭环境致病与防治教育部重点实验室,山西太原030001

出　　处：《分析科学学报》2024年第2期132-138,共7页Journal of Analytical Science

基　　金：国家自然科学基金(81803286);中国博士后科学基金(2015M571286);山西医科大学校创新基金(01201503)。

摘　　要：生物硫醇在生物体内的代谢过程中起着重要作用,如体内半胱氨酸(Cys)和谷胱甘肽(GSH)含量异常会引发一系列疾病。为了建立一种快速、定量检测生物硫醇的荧光分析方法,本文以卡托普利(Captopril,Capt)为模板,采用湿化学还原法成功合成了发红色荧光的银纳米团簇(Capt-Ag NCs),对Capt-Ag NCs的形貌、结构、元素组成和光学性能等进行了表征。基于生物硫醇小分子对Capt-Ag NCs的荧光猝灭作用建立了定量检测Cys和GSH的新方法,在最佳实验条件下,Cys和GSH的线性范围分别为1~100μmol/L和2~180μmol/L,检出限分别为0.054μmol/L和0.23μmol/L。该方法可用于人血浆中Cys的测定,回收率为95.3%~103.3%。实验结果表明,CaptAg NCs作为一种新型信号关闭荧光探针,可用于Cys和GSH等生物硫醇的检测。Biothiols play an important role in the metabolism of organisms,such as the abnormal content of cysteine(Cys)and glutathione(GSH)in human body will lead to a series of diseases.To establish a rapid and quantitative fluorescence assay method for detecting biothiols,red fluorescent silver nanoclusters was synthesized by wet chemical reduction method using captopril(Capt)as a template(namely Capt-AgNCs).The morphology,structure,elemental composition and optical properties of Capt-AgNCs were characterized.A new method for quantitative detection of Cys and GSH was established based on the fluorescence quenching effect of biothiols on Capt-AgNCs.Under the optimal experimental conditions,the linear ranges for Cys and GSH were 1-100μmol/L and 2-180μmol/L,respectively.And the detection limits for Cys and GSH were 0.054 and 0.23μmol/L,respectively.The method could be used for the determination of Cys in human plasma with the recoveries of 95.3%-103.3%.The experimental results showed that Capt-AgNCs as a novel signal-off fluorescent probe could be used for detection of biothiols such as Cys and GSH.

关 键 词：银纳米团簇 荧光猝灭法 生物硫醇 半胱氨酸 谷胱甘肽 

分 类 号：O657.39[理学—分析化学]