全自动QuEChERS-超高效液相色谱-串联质谱法测定花类代用茶中链格孢霉毒素  

Determination of Alternaria Toxins in Flower Substitute Tea by Full-automatic QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：叶梦薇 黄媛 冯丽凤 黄芊 蔡小明 梁敏 YE Mengwei;HUANG Yuan;FENG Lifeng;HUANG Qian;CAI Xiaoming;LIANG Ming(National Quality Supervision and Testing Center for Processed Food,Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002)

机构地区：[1]国家加工食品质量检验检测中心,福建省产品质量检验研究院,福建福州350002

出　　处：《分析科学学报》2024年第2期172-178,共7页Journal of Analytical Science

基　　金：福建省产品质量检验研究院科技项目(KY202114A);福建省市场监督管理局重点科技项目(FJMS2023074)。

摘　　要：本文建立了全自动Qu ECh ERS-超高效液相色谱-串联质谱法快速测定花类代用茶中5种链格孢霉毒素的分析方法。样品经全自动Qu ECh ERS前处理,以ACQUITY UPLCHSST3色谱柱分离,在电喷雾离子源负源模式下,进行多反应监测分析。在优化后的条件下,5种链格孢霉毒素在各自的线性响应范围内线性关系良好,相关系数(r)均大于0.999,在6种花类代用茶基质的3个不同加标水平下平均回收率为82.3%~105.7%,相对标准偏差(RSD)小于10%,方法检出限为0.5~1.5μg/kg。方法具有分析速度快、稳定性好、灵敏度高的特点,能够满足花类代用茶中链格孢霉毒素的快速检测需求。A method for the simultaneous determination of 5 kinds of alternaria toxins in flower substitute tea was established by ultra performance liquid chromatography-tandem mass spectrometry combined with full-automatic QuEChERS extraction.The samples were pre-treated by full-automatic QuEChERS experiment instrument,separated by ACQUITY UPLC HSS T 3 column and detected by MS/MS system under negative electro-spray ionization(ESI-)mode with multiple reaction ion monitoring(MRM).Under the optimized conditions,all the 5 kinds of alternaria toxins were well separated with good linear relationships within their respective linear ranges,and the correlation coefficients(r)were greater than 0.999.The average recoveries of 5 kinds of alternaria toxins in 6 flower substitute tea matrices at 3 kinds of spiked levels ranged from 82.3%to 105.7%,with relative standard deviations no more than 10%,and the detection limits were between 0.5-1.5μg/kg.The method has characteristics of fast analysis speed,high sensitivity and accuracy,and it can be used for the rapid detection of alternaria toxins in flower substitute tea.

关 键 词：超高效液相色谱-串联质谱法 花类代用茶 链格孢霉毒素 全自动QuEChERS 

分 类 号：O657.63[理学—分析化学]