固相萃取-一标多测高效液相色谱法测定化妆品中5种苯并三唑类防晒剂  

Quantitative Analysis of Multi-components by a Single Marker for Five Benzotriazole Sunscreens in Cosmetics Using Solid-Phase Extraction Coupled with High Performance Liquid Chromatography

作　　者：王磊[1,2] 吴越[1] WANG Lei;WU Yue(Department of Food and Environmental Engineering,Chuzhou Polytechnic,Chuzhou 239000;School of Chemistry and Chemical Engineering,Anhui University,Hefei 230601)

机构地区：[1]滁州职业技术学院食品与环境工程学院,安徽滁州239000 [2]安徽大学化学化工学院,安徽合肥230601

出　　处：《分析科学学报》2024年第2期225-230,共6页Journal of Analytical Science

基　　金：安徽省高校自然科学研究重点项目(KJ2021A1406);安徽省高校优秀青年骨干教师国内访问研修项目(gxgnfx2021201)。

摘　　要：建立了固相萃取技术结合高效液相色谱测定化妆品中5种苯并三唑类防晒剂的一标多测定量分析方法。该方法待测样品使用HLB固相萃取小柱净化处理,以亚甲基双-苯并三唑基四甲基丁基酚为内参物,通过建立该物质与另外4种物质的相对校正因子,计算出各物质的含量。研究了不同进样量、不同流动相流速及不同柱温对相对校正因子的影响,比较了一标多测法和外标法的计算结果。结果表明,5种物质的线性关系良好(R^(2)≥0.9997),检测限和定量限分别为4.00~26.43μg/L和10.00~60.42μg/L;各物质的相对校正因子重复性较好,其相对标准偏差(RSD)为0.25%~1.16%;两种方法计算结果无明显差异,其相对平均偏差(RAD)为0.12%~2.19%。该方法操作简单,使用标准物质少,检测效率高,可用于化妆品中5种防晒剂的含量测定和质量控制。A quantitative analysis of multi-components by a single marker(QAMS)for five benzotriazole sunscreens in cosmetics using solid-phase extraction(SPE)coupled with HPLC was established.The samples to be tested were purified by HLB SPE column,and the content of other substances was calculated by establishing the relative correction factor between the substance and the other four substances with Bisoctrizole as the internal reference.The effects of different injection volume,different flow rate of mobile phase and different column temperature on the relative correction factor were studied,and the calculation results of QAMS and the external standard method(ESM)were compared.The results showed that the five substances had a good linear relationship(R^(2)≥0.9997),and the detection limit and quantitative limit were 4.00-26.43μg/L and 10.00-60.42μg/L,respectively.The relative correction factor had good repeatability,and the relative standard deviation were 0.25%-1.16%.There was no significant difference between QAMS and ESM,and the relative average deviation were 0.12%-2.19%.The established QAMS method with simple operation,less standard substances and high detection efficiency,which can be used for the content determination and quality control of five sunscreens in cosmetics.

关 键 词：高效液相色谱 固相提取 一标多测 苯并三唑 防晒剂 化妆品 

分 类 号：O6577.2[理学—分析化学]