超声提取-液相色谱-串联质谱法检测纺织品中碱性黄2含量  

Determination of Basic Yellow 2 in Textiles by Liquid Chromatography-Tandem Mass Spectrometry with Ultrasonic Extraction

作　　者：潘建君 张晓婷 王栋 冯云 闵洁 PAN Jianjun;ZHANG Xiaoting;WANG Dong;FENG Yun;MIN Jie(Huzhou Inspection and Quarantine Comprehensive Technical Service Center,Huzhou 313000;Shaoxing Customs Comprehensive Technical Service Center,Shaoxing 312099;Ningbo Customs Technical Center,Ningbo 315012;Key Laboratory of Science and Technology,Ministry of Education,College of Chemistry and Chemical Engineering,Donghua University,Shanghai 201620)

机构地区：[1]湖州检验检疫综合技术服务中心,浙江湖州313000 [2]绍兴海关综合技术服务中心,浙江绍兴312099 [3]宁波海关技术中心,浙江宁波315012 [4]生态纺织教育部重点实验室,东华大学化学与化工学院,上海201620

出　　处：《分析科学学报》2024年第2期239-243,共5页Journal of Analytical Science

基　　金：浙江省自然科学基金(LTGC23B050001)。

摘　　要：建立了超声提取-液相色谱-串联质谱测定纺织品中碱性黄2含量的方法。样品经甲醇超声提取后,利用液相色谱-串联质谱法进行测试分析。方法采用C18(150mm×2.1mm,5μm)色谱柱,以甲醇(A)-0.1%甲酸-5mmol/L乙酸铵水溶液(B)为流动相进行梯度洗脱,正离子多反应监测(MRM)模式检测。结果表明,在1.0~100.0μg/L浓度范围内,碱性黄2的线性相关系数R^(2)为0.9997;方法检出限(LOD)和定量限(LOQ)分别为0.006mg/kg和0.02mg/kg;加标回收率为82.0%~106.0%,相对标准偏差(RSD,n=6)为1.08%~7.31%。本方法操作简单,加标回收率高,重复性好,适用于纺织品中碱性黄2的测定。A liquid chromatography-tandem mass spectrometry(LC-MS/MS)with ultrasonic extraction method was developed for the determination of basic yellow 2 in textiles.The textiles samples were extracted with methanol by ultrasonication,and basic yellow 2 was quantified by LC-MS/MS.The samples were detected in positive ion reaction monitoring MRM mode.A C18 column(2.1 mm×150 mm,5μm)was used with a gradient elution using methanol(A)-0.1%formic acid-5 mmol/L ammonium acetate aqueous solution(B)as the mobile phase.The results showed that the linearity of basic yellow 2 was good in the range of 1.0-100.0μg/L with the R^(2)=0.9997;the limits of detection and limits of quantification were 0.006 mg/kg and 0.02 mg/kg,respectively.The average recoveries were 82.0%-106.0%,and the relative standard deviations(RSDs)were 1.08%-7.31%(n=6).The method is suitable for the determination of basic yellow 2 in textiles.

关 键 词：纺织品 碱性黄2 液相色谱-串联质谱 超声提取 

分 类 号：O657.63[理学—分析化学] TS197[理学—化学]