毛果杨MGT基因家族全基因组水平鉴定及表达分析  

Genome-wide Identification and Expression Analysis of the MGT Gene Family in Populus trichocarpa

作　　者：栾雪 张洋[1] 王瑞琪 刘聪[1] 姜廷波[1] 魏志刚 Luan Xue;Zhang Yang;Wang Ruiqi;Liu Cong;Jiang Tingbo;Wei Zhigang(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,150040;Research Center of Saline and Alkali Land of State Forestry and Grassland Administration,Chinese Academy of Forestry,Beijing,100091)

机构地区：[1]东北林业大学,林木遗传育种国家重点实验室,哈尔滨150040 [2]中国林业科学研究院,国家林业和草原局盐碱地研究中心,北京100091

出　　处：《分子植物育种》2024年第9期2785-2792,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31770640);中央级公益性科研院所基本科研业务费专项(CAFYBB2020ZA005)共同资助。

摘　　要：为探究MGT基因家族在毛果杨生长发育及响应胁迫中的作用,本研究在毛果杨全基因组范围,通过生物信息学分析PtrMGT基因家族成员的数量、进化关系、染色体位置、基因结构、编码蛋白基本特征、启动子顺式作用元件,并对各成员的组织表达特异性以及对NaCl胁迫与ABA处理的响应特性进行了分析。结果表明:PtrMGT家族共含有10个基因,可分为4个亚族;有2对同源基因且Ka/Ks值均小于1;家族成员启动子区含有数量不等的非生物胁迫响应元件,共12种、146个元件;PtrMGT家族编码的蛋白均含有GMN序列,同一亚族基因编码蛋白的序列相对保守;PtrMGT家族成员在毛果杨根、茎和叶表达量具有差异;NaCl胁迫下,PtrMGT家族基因在根、茎和叶中表达量在0~48 h内均表现为先上升后下降的变化趋势;ABA处理下,大部分成员表达量先升后降,小部分成员表达量持续下降,表明PtrMGT家族基因对NaCl胁迫与ABA处理产生响应且响应模式出现分化。本研究为深入研究PtrMGT基因家族功能提供理论基础。To explore the role of MGT gene family in growth and development and stress response of poplar,in this study,the number,evolutionary relationship,chromosomal location,gene structure,basic characteristics of coding proteins and cis acting elements of promoter of PtrMGT gene family members were identified by bioinformatics method in the whole genome of Populus trichocarpa.The specificity of tissue expression and the response to NaCl stress and ABA treatment.The results showed that the PtrMGT family contained 10 genes,which could be divided into 4 subfamilies.There were 2 pairs of paralogous genes and the Ka/Ks value was less than 1.The promoter regions of family members contained 12 species and 146 elements,which were different in number of abiotic stress response elements.Proteins encoded by the PtrMGT family all contained GMN,and the sequence of proteins encoded by the same subfamily gene was relatively conservative.The expression levels of PtrMGT family members were different in roots,stems and leaves.Under NaCl stress,the expression levels of PtrMGT family genes in roots,stems and leaves were firstly increased and then decreased during 0~48 h.Under ABA treatment,the expression of PtrMGT family genes was differentiated,and the expression of most members increased first and then decreased,while the expression of a small number of members continued to decrease.These results indicated that ptrMGT family genes were responsive to NaCl stress and ABA treatment,and the response patterns were differentiated.This study provides a theoretical basis for further revealing the function of PtrMGT gene family.

关 键 词：毛果杨 MGT 生物信息学分析 盐胁迫 ABA处理 

分 类 号：S792.11[农业科学—林木遗传育种]