矮腥黑粉菌胁迫的小麦全基因组重测序与转录组联合分析  

Combined Analysis of Whole Genome Re-sequencing and Transcriptome of Wheat Under the Stress of Tilletia controversa Kühn

作　　者：沈潼 肖越 粟神强 贾玉凤 郭巧玲 刘琦 肖永贵[2] Shen Tong;Xiao Yue;Su Shenqiang;Jia Yufeng;Guo Qiaoling;Liu Qi;X iao Yonggui(Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests,College of Agriculture,Xinjiang Agricultural University,Urumqi,830052;National Wheat Improvement Center,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing,100081)

机构地区：[1]新疆农业大学农学院,农林有害生物监测与安全防控重点实验室,乌鲁木齐830052 [2]中国农业科学院作物科学研究所国家小麦改良中心,北京100081

出　　处：《分子植物育种》2024年第9期2793-2801,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31860477);中国农业科学院作物科学研究所-新疆农业大学合作项目(2519HXKT3)共同资助。

摘　　要：为揭示矮腥黑粉菌胁迫下的小麦抗感品种间遗传背景差异,筛选小麦抗矮腥黑穗病候选基因。本研究以小麦感病品种‘伊犁053’和中抗品种‘中麦175’为研究对象,在接种矮腥黑粉菌后对籽粒组织进行重测序与转录组测序的联合分析。经过差异InDel分析后,共有69840个变异基因与转录组数据进行了联合分析,有7300个基因在小麦发病籽粒组织中有表达,有162个(2.22%)基因呈极显著差异表达,且基因表达量倍数变化≥1.5倍(‘中麦175’相对于‘伊犁053’,有125个上调,37个下调);经过差异SNP分析后,共有98331个变异基因与转录组数据进行了联合分析,有7438个基因在小麦发病籽粒组织中有表达,有126个(1.69%)基因呈极显著差异表达,且基因表达量倍数变化≥1.5倍(‘中麦175’相对于‘伊犁053’,有86个上调,40个下调)。本研究成功揭示了在矮腥黑穗病胁迫下抗感病小麦品种间的遗传差异表达信息,为进一步筛选小麦抗矮腥黑穗病候选基因提供参考。In order to reveal the difference of genetic background between susceptible cultivar(Yili053)and resistant cultivar(Zhongmai175)under the stress of Tilletia controversa Kühn(TCK),and to screen the candidate genes of resistance to dwarf bunt,the study used the combined analysis of whole genome re-sequencing and transcriptom of kernels on two wheat cultivars,which were inoculated by TCK to evaluate the effect.After differential InDel analysis,a total of 69840 mutant genes were analyzed with transcriptome data,including 7300 genes were expressed in wheat,and 162(2.22%)of the genes were significantly differential expressed,the fold change by more than 1.5 times(125 up-regulated and 37 down-regulated for'Zhongmai175'compared with Yili053);After differential SNP analysis,a total of 98331 mutant genes were analyzed with transcriptome data,including7438 genes were expressed in wheat,and 126(1.69%)of the genes were significantly differential expressed,the fold change by more than 1.5 times(86 up-regulated and 40 down-regulated for'Zhongmai175'compared with Yili053).The genetic differential expression information and the genes with significant variation were successfully screened out,which lays a foundation for the further work of screening candidate genes for resistance to dwarf bunt in wheat.The results might provide a scientific basis for disease resistance breeding of wheat in Xinjiang Uygur Autonomous Region.

关 键 词：小麦矮腥黑穗病 全基因组重测序 转录组 联合分析 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]