油茶Aux/IAA基因家族的鉴定与分析  

Identification and Analysis of AUX/IAA Gene Family in Camellia oleifera

作　　者：张廷忠 梁康 陈荟 朱亚艳 许杰 Zhang Tingzhong;Liang Kang;Chen Hui;Zhu Yayan;Xu Jie(Guizhou Academy of Forestry,Guiyang,550005)

机构地区：[1]贵州省林业科学研究院,贵阳550005

出　　处：《分子植物育种》2024年第9期2847-2855,共9页Molecular Plant Breeding

基　　金：贵州本地油茶良种选育项目(特林研2020-15);贵州油茶团队服务企业行动计划项目(黔科合服企[2018]4003);贵州省油茶工程技术研究中心项目(黔科合平台人才[2018]5252)共同资助。

摘　　要：为了获取油茶Aux/IAA基因家族的分布和结构特征,利用HMM进行油茶全基因组Aux/IAA基因的鉴定。结果表明,油茶Aux/IAA基因非均匀分布在10条染色体上;序列长度和外显子数量差异较大,氨基酸长度范围在132~1085之间,外显子数量为2~16个,同一系统发育亚组上的基因具有类似的外显子数量和基序结构;油茶Aux/IAA部分基因具备响应生长素的顺式作用元件外,还具备其他激素响应的基序和光响应元件。转录组数据表明,CoIAAChr12_3、CoIAAChr7_5、CoIAAChr9_3、CoIAAChr9_4、CoIAAChr7_6基因在油茶花芽、花瓣、萼片、雄蕊、雌蕊中高表达,可能与花的形成和发育相关。CoIAAChr12_5在油茶种子中高表达,可能与油茶种子发育相关。本研究为Aux/IAA基因家族在油茶中的系统研究提供了基础。In order to obtain the distribution and structural characteristics of Camellia oleifera Aux/IAA gene family,HMM was used to identify Aux/IAA genes in the whole genome of C.oleifera.The results showed that C.oleifera Aux/IAA genes were unevenly distributed in 10 chromosomes;the sequence length and the number of exons vary greatly,the amino acid length ranged from 132 to 1085,the number of exons was 2 to 16,genes on the same phylogenetic subgroup had similar exon numbers and motif structures;some Aux/IAA genes of C.oleifera had cis-acting elements in response to auxin,as well as other hormone-responsive motifs and light-responsive elements.Transcriptome data showed that CoIAAChr12_3,CoIAAChr7_5,CoIAAChr9_3,CoIAAChr9_4,CoIAAChr7_6 genes were highly expressed in C.oleifera flower buds,petals,sepals,stamens,and pistils,which were related to flower formation and development.Co IAAChr12_5 was highly expressed in seeds,which was associated with seed development.This study provided the basis for the systematic study of Aux/IAA gene family in C.oleifera.

关 键 词：油茶 基因家族 AUX/IAA 启动子元件分析 基因表达 

分 类 号：S794.4[农业科学—林木遗传育种]