胡桃楸激素调控相关JmDELLA基因克隆与表达分析  

Cloning and Expression Analysis of JmDELLA Gene Related to Juglans mandshurica Hormone Regulation

作　　者：范新蕊 秦柏婷 蔡佳友 傅靖棋 董天一 张丽杰[1] Fan Xinrui;Qin Baiting;Cai Jiayou;Fu Jingqi;Dong Tianyi;Zhang Lijie(Key Laboratory of Forest Tree Genetics,Breeding and Cultivation of Liaoning Province,College of Forestry,Shenyang Agricultural University,Shenyang,110866)

机构地区：[1]沈阳农业大学林学院,辽宁省林木遗传育种与培育重点实验室,沈阳110866

出　　处：《分子植物育种》2024年第9期2891-2897,共7页Molecular Plant Breeding

基　　金：辽宁省教育厅重点攻关项目(LSNZ201905);国家重点研发计划项目(2017YFD0600605)共同资助。

摘　　要：为探讨胡桃楸激素调控相关JmDELLA基因在花芽性别分化过程中的调控机理,本研究从胡桃楸转录组测序数据库中筛选出与激素调控相关DELLA基因的差异片段,通过PCR扩增技术获得DELLA基因CDS全长序列,命名为JmDELLA(GenBank登录号:400411)。并利用生物信息学软件对JmDELLA基因进行生物信息学分析,利用RT-PCR对胡桃楸不同器官组织进行表达分析。克隆获得JmDELLA基因的CDS序列全长为1842 bp,编码613个氨基酸,将该基因编码的氨基酸序列比对和聚类分析,结果表明该基因与核桃(Juglans regia)、薄皮山核桃(Carya illinoinensis)、杨梅(Morella rubra)亲缘关系同源性可以达到80%以上。RT-PCR表达分析表明胡桃楸激素调控相关JmDELLA基因在雌、雄花芽中表达量最高,因此推测该基因参与了胡桃楸花芽性别分化的整个过程。该结果为深入解析胡桃楸雌雄异型异熟性别分化的分子调控机理提供理论依据。In order to explore the regulation mechanism of hormone regulation related JmDELLA gene in the process of flower bud gender differentiation,the differential fragments of hormone regulation related DELLA gene were screened from the transcriptome sequencing database of Juglans mandshurica,and the full-length CDS sequence of DELLA gene was obtained by PCR amplification,named JmDELLA(GenBank accession number:400411).Bioinformatics software was used to bioinformatics analyze JmDELLA gene,and RT-PCR was used to analyze the expression of different organs and tissues of Juglans mandshurica.The total length of the CDs sequence of JmDELLA gene was 1842 bp in length and encoding 613 amino acids.The results of amino acid sequence alignment and cluster analysis showed that the gene had more than 80%homology with Juglans regia,Pecan(Carya illinoinensis)and bayberry(Morella rubra).RT-PCR expression analysis showed that the JmDELLA gene related to the hormone regulation of Juglans mandshurica has the highest expression in female and male flower buds.Therefore,it is speculated that this gene is involved in the whole process of sexual differentiation of Juglans mandshurica flower buds.This result provides a theoretical basis for in-depth analysis of the molecular regulation mechanism of the heterogeneous sex differentiation of Juglans mandshurica.

关 键 词：胡桃楸 性别分化 JmDELLA 基因克隆 表达分析 

分 类 号：S792.132[农业科学—林木遗传育种]