普通野生稻对南方水稻黑条矮缩病的抗性机制分析及主效QTL定位  

Analysis of the Resistance Mechanism and Major QTL of Common Wild Rice to Southern Rice Black-streaked Dwarf Disease

作　　者：农保选 郭辉 张宗琼 夏秀忠 杨行海 曾宇 崔丽贤[2] 梁云涛 秦碧霞[2] 李丹婷 Nong Baoxuan;Guo Hui;Zhang Zongqiong;Xia Xiuzhong;Yang Xinghai;Zeng Yu;Cui Lixian;Liang Yuntao;Qin Bixia;Li Dantingg(Guangxi Key Laboratory of Rice Genetics and Breeding,Rice Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,530007;Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,530007)

机构地区：[1]广西壮族自治区农业科学院水稻研究所,广西水稻遗传育种重点实验室,南宁530007 [2]广西壮族自治区农业科学院植物保护研究所,广西作物病虫害生物学重点实验室,南宁530007

出　　处：《分子植物育种》2024年第9期2930-2937,共8页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2018YFD0200302);广西自然科学基金项目(2018GXNSFAA138124);广西科技计划项目(桂科AD18281067);广西农业科学院科技发展基金项目(桂农科2020ZX13);广西水稻优质化育种研究人才小高地项目(水稻人才小高地QN-20)共同资助。

摘　　要：南方水稻黑条矮缩病给水稻生产带来巨大的产量损失,开展水稻对该病的抗性机制分析及抗性基因定位,将为抗病品种培育提供材料基础和理论依据。本研究利用QTL-seq技术结合连锁分析定位抗病野生稻材料Y11的抗性主效QTL。结果表明,Y11对南方水稻黑条矮缩病的抗性源于对病毒的抗性而非抗虫性,属于数量性状。此外,在第6染色体上SSR标记RM20148和RM20297之间检测到一个新的南方水稻黑条矮缩病抗性主效QTL qSRBSDV6。本研究初步阐明了普通野生稻对南方水稻黑条矮缩病的抗性机制,定位到一个新的南方水稻黑条矮缩病抗性主效QTL。研究结果将为南方水稻黑条矮缩病的分子育种奠定基础。Southern rice black-streaked dwarf disease(SRBSDD),has caused severe yield loss in rice production.To analyse the resistance mechanism of common wild rice Y11 and the major QTLs for resistance to SRBSDD,here,antixenosis test,antibiosis test,artificial inoculation,and QTL-seq technique combined with linkage analysis were used.The results showed that the resistance to SRBSDD of Y11 was derived from the resistance to virus rather than insect,and expressed the genetic characteristics of quantitative characters.A new major QTL qSRBSDV6 for resistance to SRBSDD was mapped between SSR molecular markers RM20148 and RM20297 on chromosome 6.In this study,the resistance characteristics of common wild rice to SRBSDD was preliminarily revealed,and a new major QTL for SRBSDD resistance was mapped,the research results will lay the foundation for the molecular breeding of SRBSDD.

关 键 词：南方水稻黑条矮缩病 普通野生稻 抗性 QTL 分子标记 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]