基于KASP标记快速鉴定瓠瓜杂种F_(1)纯度的方法  

A KASP-Based Rapid Procedure for Bottle Gourd Hybrid F_(1) Seed Purity Survey

作　　者：冯子珊 吴晓花[2] 李艳伟 鲁忠富[2] 王尖 吴新义[2] 汪宝根[2] 叶子弘 李国景[2] 汪颖 Feng Zishan;Wu Xiaohua;Li Yanwei;Lu Zhongfu;Wang Jian;Wu Xinyi;Wang Baogen;Ye Zihong;Li Guojing;Wang Ying(College of Life Science,China Jiliang University,Hangzhou,310018;Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021)

机构地区：[1]中国计量大学生命科学学院,杭州310018 [2]浙江省农业科学院蔬菜研究所,杭州310021

出　　处：《分子植物育种》2024年第9期2947-2951,共5页Molecular Plant Breeding

基　　金：浙江省基础公益研究计划项目(LGN19C150011);浙江省“十四五”蔬菜新品种选育重大科技专项(2021C0-2065);瓠瓜高质量种子生产关键技术研究项目(2021C0204-2)共同资助。

摘　　要：瓠瓜为中国南方重要特色瓜类蔬菜之一,随着瓠瓜育成品种数量逐年增多,同名异种、同种异名引发的知识产权纠纷多见。传统上对品种的鉴定主要依靠田间种植,效率低。本研究以浙江省农业科学院蔬菜所新育成的高品质短棒瓠瓜杂交种‘浙蒲9号’及其亲本为试材,选取了3对‘浙蒲9号’亲本特异性SNP引物,基于LGC KASP高通量技术平台,对‘浙蒲9号’父本、母本以及92份F_(1)材料进行鉴定,结果显示‘浙蒲9号’F_(1)种子纯度为97.8%,与田间种植纯度鉴定结果一致。该方法准确性好、成本低且高效,单孔不超过1元,检测全程仅需5 d左右,大大缩短了品种鉴定的时间,是一种准确、简便、快速的瓠瓜品种鉴定和纯度检测方法,应用前景非常广泛。Bottle gourd is one of the impor tant distinctive vegetables in China.At present,there are many varieties of bottle gourds in the market,and disputes of the same name,different names of the same thing and intellectual property rights often occur.Traditional identification of varieties mainly depends on low efficiency field planting.In this study,‘Zhepu No.9’,a new variety with high-quality and short straight fruit shape,and its parents were used as test materials,and 3 pairs of parent-specific SNP primers of'Zhepu No.9'were selected based on LGC KASP high-throughput technology platform.The results showed that the purity of F_(1) seeds of‘Zhepu No.9’was 97.8%,which was consistent with the field purity identification.The method has advantages of good accuracy,low cost and high efficiency.The expense of single hole does not exceed 1 RMB,and the whole detection procedure only takes about 5 days,which greatly shortens the time for variety identification.This study provided a precise,rapid and convenient KASP-based procedure for bottle gourd seed purity survey with a broad application prospect.

关 键 词：瓠瓜 KASP标记 纯度鉴定 快速 浙蒲9号 

分 类 号：S642.9[农业科学—蔬菜学]