大豆GmHMGS基因的鉴定及表达模式分析  

Identification and Expression Pattern Analysis of GmHMGS Gene in Soybean

作　　者：娄银 高浩竣 王茜 牛景萍 王敏[1] 杜维俊[1] 岳爱琴[1] LOU Yin;GAO Hao-jun;WANG Xi;NIU Jing-ping;WANG Min;DU Wei-jun;YUE Ai-qin(College of Agriculture,Shanxi Agricultural University,Taigu 030801;College of Life Sciences,Shanxi Agricultural University,Taigu 030801)

机构地区：[1]山西农业大学农学院,太谷030801 [2]山西农业大学生命科学学院,太谷030801

出　　处：《生物技术通报》2024年第4期110-121,共12页Biotechnology Bulletin

基　　金：山西省科技重大专项计划揭榜挂帅项目子课题(202201140601025-3-06);山西省科技成果转化引导专项计划(202104021301038);中央引导地方科技发展资金项目(YDZJSX2022A035);山西农业大学科技创新基金项目(2018YJ29);山西省研究生教育创新项目(2023SJ106)。

摘　　要：【目的】3-羟基-3-甲基戊二酰辅酶A合酶(HMGS)是甲羟戊酸途径中的一个关键酶,在植物生长发育和萜类化合物的合成中起着重要作用。对GmHMGS基因进行生物信息学和表达模式分析,为探究GmHMGS基因的功能奠定基础。【方法】运用生物信息学方法对该基因进行鉴定和分析,采用实时荧光定量PCR(RT-qPCR)对该基因表达模式进行分析。【结果】GmHMGS基因具有6个家族成员,命名为GmHMGS1-GmHMG6。理化性质和基因结构分析表明,GmHMGS3、GmHMG4、GmHMGS5、GmHMGS6为稳定的亲水性蛋白,GmHMGS都具有HMG-CoA合酶的保守结构域。启动子序列分析发现GmHMGS具有激素及逆境胁迫相关作用元件。组织表达模式分析发现,GmHMGS在根、茎、叶、花、籽粒、荚皮,根瘤中均有表达,GmHMGS1、GmHMG2、GmHMG3、GmHMG5、GmHMG6在叶片中的相对表达量较高,GmHMGS4在根中的相对表达量最高。非生物胁迫和不同外源激素诱导条件下表达分析表明,GmHMGS基因对PEG6000、NaCl、H_(2)O_(2)胁迫以及MeJA、ABA外源激素均有响应,且GmHMGS1和GmHMGS6在多种逆境胁迫下的相对表达量较高。【结论】GmHMGS基因家族可能参与大豆抗旱、耐盐和抗氧化胁迫的应答。【Objective】3-hydroxy-3-methylglutaryl-CoA synthase(HMGS)is a key enzyme in the mevalonate pathway and plays an important role in the growth,development and synthesis of terpenoids.Bioinformatics study and expression pattern analysis of the GmHMGS gene can lay the foundation for exploring the function of GmHMGS gene.【Method】The gene was identified and analysed using bioinformatics approaches,and then its expression pattern was examined using real-time fluorescence quantitative PCR(RT-qPCR).【Result】The GmHMGS gene had six family members in total,named GmHMGS1 to GmHMG6.The analysis of physicochemical properties and gene structure showed that GmHMGS3,GmHMG4,GmHMGS5,GmHMGS6 were stable hydrophilic proteins,and all GmHMGS possessed conserved functional domains of HMG-CoA synthase.Promoter sequence analysis revealed that GmHMGS has hormone and adversity stress related action elements.The analysis of tissue expression pattern revealed that GmHMGS was expressed in roots,stems,leaves,flowers,grains,pod skins,and root nodules,with leaves having the highest relative expression of GmHMGS1,GmHMG2,GmHMG3,GmHMG5,and GmHMG6,and roots having the highest relative expression of GmHMGS4.The GmHMGS genes were responsive to PEG6000,NaCl,and H_(2)O_(2) stresses,as well as MeJA and ABA exogenous hormones,and the relative expression of GmHMGS1 and GmHMGS6 was higher under a wide range of adversity stresses,according to expression analysis under abiotic stress and different exogenous hormone-induced conditions.【Conclusion】GmHMGS gene family may be involved in response to drought,oxidative and salt stresses in soybean.

关 键 词：大豆 GmHMGS 生信分析 表达分析 胁迫处理 

分 类 号：Q943.2[生物学—植物学] S565.1[农业科学—作物学]