牛血中β2-糖蛋白的提取纯化及免疫原性研究  

Extraction,purification and immunogenicity ofβ2-glycoprotein inbovine blood

作　　者：曲超 邓庚粮 贺书楷 杨溪 张哲源 欧阳浩然 刘绍[1] QU Chao;DENG Gengliang;HE Shukai;YANG Xi;ZHANG Zheyuan;OUYANG Haoran;LIU Shao(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;Wuhan PROBIO Biotechnology,Wuhan 430223,China;Hunan Provincial Institute of Product and Goods Quality Inspection,Changsha 410007,China)

机构地区：[1]湖南农业大学食品科学技术学院,长沙410128 [2]湖北武汉博百欧生物技术有限公司,武汉430223 [3]湖南省产商品质量检验研究院,长沙410007

出　　处：《北京农学院学报》2024年第2期99-102,114,共5页Journal of Beijing University of Agriculture

摘　　要：【目的】为β2GPI的获得增加一个新的途径,提高牛血的利用效益,减少牛血资源浪费。【方法】牛血清经HClO_(4)沉淀、Heparin Sepharose CL-6B亲和层析及分子筛等方法纯化获得目的蛋白β2GPI。利用凝胶电泳技术对提取的Bβ2GPI进行了纯度鉴定和分析,然后采用ELISA技术对Bβ2GPI的免疫原性进行了评估。【结果】试验表明提取纯化的Bβ2GPI纯度约为95%,其反应性与β2GPI标准品相同。【结论】从牛血中成功提取纯化β2GPI,且所获得的蛋白具有较高纯度和良好的免疫原性。[Objective]To add a new way to the acquisition of Bβ2GPI,improve the utilization value of bovine blood,and improve the waste of bovine blood resources to a certain extent.[Methods]Initial precipitation with perchloric acid was followed by further purification using Heparin Sepharose CL-6B affinity chromatography and zeolite.The purity of the extracted Bβ2GPI was identified and analyzed by gel electrophoresis,and then the immunogenicity of Bβ2GPI was evaluated by Enzyme-linked immunosorbent assay(ELISA).[Results]Experiments showed that the purity of the extracted and purified Bβ2GPI was about 95%,and its reactivity was the same as that of theβ2GPI standard.[Conclusion]Bβ2GPI was successfully extracted and purified from bovine blood,and the obtained protein had high purity and good immunogenicity.

关 键 词：牛血 β2-糖蛋白 提取 纯化 免疫原性 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]