生长分化因子11对股动脉介入损伤术后小鼠血管的修复作用  

Effects of Growth Differentiation Factor 11 on Vascular Repair After Femoral Artery Interventional Injury in Mice

作　　者：李涛 侯义江 程杨阳 张佳佳 LI Tao;HOU Yijiang;CHENG Yangyang;ZHANG Jiajia(Department of Endocrinology,General Hospital of Central Theater Command,Wuhan Hubei 430070,China)

机构地区：[1]中部战区总医院内分泌科,湖北武汉430070

出　　处：《联勤军事医学》2024年第2期95-98,共4页Military Medicine of Joint Logistics

基　　金：国家自然科学基金项目(82000776)。

摘　　要：目的 探讨生长分化因子11(growth differentiation factor 11,GDF11)对股动脉介入损伤术后小鼠血管的修复作用。方法 利用导丝损伤的方法构建C57BL/6小鼠股动脉损伤模型,将30只小鼠随机分为对照组、模型组和重组人GDF11蛋白处理组(GDF11组),每组10只。干预14天时,采用苏木精-伊红(hematoxylin-eosin,HE)染色检测各组小鼠股动脉内膜增生情况,同时采用免疫荧光检测各组小鼠损伤段血管CD31表达水平,利用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测各组小鼠外周血血清中血管内皮生长因子(vascular endothelial growth factor,VEGF)、表皮生长因子(epidermal growth factor,EGF)、基质细胞衍生因子1(stromal-derived factor-1,SDF-1)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的含量变化。结果 与对照组比较,模型组股动脉损伤小鼠外周血中促血管修复因子VEGF、SDF-1、bFGF、EGF的含量明显升高(P均<0.01);损伤血管增生内膜与中膜面积比显著升高(P<0.01);模型组小鼠CD31表达明显降低(P<0.01)。与模型组相比,GDF11组小鼠促血管修复因子VEGF、SDF-1、bFGF、EGF的含量明显升高(P均<0.01);损伤段血管增生内膜与中膜面积比下降(P<0.01);CD31表达明显增多(P<0.01)。结论 GDF11能够抑制股动脉损伤小鼠损伤血管的新生内膜过度增生,促进再内皮化,从而对损伤血管发挥修复作用。Objective To investigate the effects of growth differentiation factor 11(GDF11)on vascular repair in mice after femoral artery interventional injury.Methods The femoral artery injury model of the C57BL/6 mice was established by the methods of guide wire damage,30 mice were divided into control group,model group and recombinant human GDF11 protein treatment group(GDF11group),with 10 mice in each.After 14 days of intervention,the intimal hyperplasia of femoral artery in each group was detected by hematoxylin-eosin(HE)staining,the expression of CD31 in the injured vascular segment of mice in each group was detected by immunofluorescence,the content changes of vascular endothelial growth factor(VEGF),epidermal growth factor(EGF),stromal-derived factor-1(SDF-1)and basic fibroblast growth factor(bFGF)were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,the content of vascular repair factors VEGF,SDF-1,bFGF and EGF in peripheral blood increased in model group(all P<0.01);the area ratio of proliferative intima to media of injured vessels significantly increased(P<0.01);the expression of CD31 in the model group significantly decreased(P<0.01).Compared with the model group,the contents of vascular repair factors including VEGF,SDF-1,bFGF and EGF in the GDF11 group significantly increased(all P<0.01);the area ratio of hyperplasia intima to media in the injured segment decreased(P<0.01);the expression of CD31 significantly increased(P<0.01).Conclusion GDF11 can inhibit neointimal hyperplasia and promote reendothelialization of injured vessels in mice with femoral artery injury,thus playing a role in the repair of injured vessels.

关 键 词：生长分化因子11 介入损伤 血管 再狭窄 再内皮化 

分 类 号：R543[医药卫生—心血管疾病]