单细胞RNA测序和空间转录组技术在常见血管外科疾病中的应用  

Application of single cell RNA sequencing and spatial transcriptome techniques in common vascular surgery diseases

作　　者：陶静[1] 杨毅宁 Tao Jing;Yang Yining(Heart and Panvascular Medical Diagnosis and Treatment Center,Xinjiang Uygur Autonomous Region People's Hospital,Xinjiang Key Laboratory of Cardiovascular Homeostasis and Regeneration Research,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院心脏及泛血管医学诊疗中心,新疆心脏血管稳态与再生医学研究重点实验室,乌鲁木齐830001

出　　处：《中华胸心血管外科杂志》2024年第3期179-185,共7页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：新疆维吾尔自治区自然科学基金重点项目(2022D01D22)。

摘　　要：血管外科疾病是指除心脑血管之外的全身性血管疾病, 随着社会进步和人口老龄化加剧, 发病率逐年增加。腹主动脉瘤、主动脉夹层、动脉硬化闭塞症、血栓形成和血管畸形是血管外科疾病中最为常见的几种类型, 其发生发展的分子机制尚不十分清楚。单细胞RNA测序(single-cell RNA sequencing, scRNA-seq)联合空间转录组技术(spatial transcriptomics, ST)可以在单细胞水平上识别新型或罕见细胞类型、研究细胞异质性和分析空间位置信息, 有助于探究其基因表达水平和三维重建, 为研究常见血管外科疾病发生发展机制开辟了一条新途径, 对生物医学研究产生了变革性影响。Vascular surgical diseases refer to systemic vascular diseases in addition to cardiovascular and cerebrovascular diseases.With the development of society and the intensification of population aging,the incidence is increasing year by year.Abdominal aortic aneurysm,aortic dissection,arteriosclerotic occlusive disease,thrombosis and vascular malformation are the most common types of vascular surgical diseases,and it is still unclear of the molecular mechanism of their occurrence and development.Single-cell RNA sequencing(scRNA-seq)combined with Spatial transcriptomics(ST)can identify new or rare cell types,study cell heterogeneity and analyze spatial location information at the single-cell level,which is helpful to explore the level of gene expression and three-dimensional reconstruction,opening up a new way to study the occurrence and development mechanism of common vascular surgical diseases,and has a transformative impact on biomedical research.

关 键 词：外科疾病 RNA 单细胞 

分 类 号：R654.3[医药卫生—外科学]