紫花苜蓿红色茎秆花青素苷含量及相关合成酶活性的研究  

Study on the Content of Anthocyanin and the Activities of Related Synthetic Enzymes in Red Stems of Alfalfa

作　　者：宗亚倩 段玉雪 匡倩 何承刚[1] 姜华[1] ZONG Yaqian;DUAN Yuxue;KUANG Qian;HE Chenggang;JIANG Hua(Faculty of Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《中国草地学报》2024年第4期62-69,共8页Chinese Journal of Grassland

基　　金：云南省农业联合基金重点项目(202101BD070001-026);云南省产业创新人才项目(2022);洱源县饲草产业科技特派团(202304BI090008)。

摘　　要：为研究空间诱变紫花苜蓿茎秆呈红色的原因,以空间诱变的紫花苜蓿红色茎秆和未诱变的紫花苜蓿绿色茎秆(CK)为材料,采用超高效液相色谱串联质谱(UPLC-MS/MS)技术测定茎秆中花青素苷成分和含量,用紫外可见分光光度计和ELISA试剂盒进行酶活性测定,并分析各花青素苷与酶活性之间的相关性。结果表明,紫花苜蓿红色茎秆中矢车菊素、飞燕草素、天竺葵素、芍药花素及总花青素苷含量显著高于绿色茎秆(P<0.05)。同时,红色茎秆中苯丙氨酸解氨酶(PAL)、肉桂酸4-羟基化酶(C4H)、查尔酮合成酶(CHS)、类黄酮-3-羟化酶(F3H)、谷胱甘肽S-转移酶(GST)活性显著高于绿色茎秆(P<0.05),且矢车菊素、飞燕草素、天竺葵素、芍药花素和总花青素苷含量与PAL、C4H、CHS、F3H和GST活性呈显著或极显著正相关(P<0.05或P<0.01)。综上,花青素苷的种类和含量可能是紫花苜蓿茎秆呈红色的主要原因,PAL、C4H、CHS、F3H和GST活性的提高促进了紫花苜蓿茎秆由绿色变为红色。In order to study the reason for the red color of space mutated alfalfa stems,the red stems of space mutated alfalfa and the green stems of unmutated alfalfa(CK)were used as research materials.The anthocyanin components and content in the stems were detected using ultra-performance liquid chromatographytandem mass spectrometry(UPLC-MS/MS),and enzyme activity was measured using UV visible spectro-photometry and ELISA kit.The correlation between each anthocyanin glycoside and enzyme activity was analyzed.The results showed that the content of cyanidins,delphinidins,pelargonidin,peonidin and total anthocyanin in the red stems of alfalfa were significantly higher than those in green stems(P<0.05).Meanwhile,the activities of phenylalanine ammonia-lyase(PAL),cinnamate 4-hydroxylase(C4H),chalcone synthase(CHS),flavonoid-3-hydroxylase(F3H),and glutathione-S-transferase(GST)in the red stems of alfalfa were significantly higher than those in green stems(P<0.05),and the contents of cyanidin,delphinidin,pelargonidin,peonidin and total anthocyanin were significantly or extremely significantly positively correlated with PAL,C4H,CHS,F3H and GST activities(P<0.05 or P<0.01).In conclusion,the type and content of anthocyanins may be the main reason for the red color of alfalfa stems.The increase in PAL,C4H,CHS,F3H,and GST enzyme activity promoted the transformation of alfalfa stems from green to red.

关 键 词：紫花苜蓿 空间诱变 花青素苷 关键酶活性 

分 类 号：S541.1[农业科学—作物学]