宏基因组二代测序技术在血液病患者合并感染诊疗中的应用  

Application of Metagenomic Next-generation Sequencing Technology to the Diagnosis and Treatment of Hematologic Diseases Complicated with Infection

作　　者：封韬[1] 付堃[1] 张智慧 芦小单[2] 高鹏[1] FENG Tao;FU Kun;ZHANG Zhihui;LU Xiaodan;GAO Peng(Department of Hematology,Jilin Provincial People’s Hospital,Changchun 130021,China;Precision Molecular Medicine Center,Jilin Provincial People’s Hospital,Changchun 130021,China)

机构地区：[1]吉林省人民医院血液科,吉林长春130021 [2]吉林省人民医院精准分子医学中心,吉林长春130021

出　　处：《长春师范大学学报》2024年第4期87-91,共5页Journal of Changchun Normal University

基　　金：吉林省卫生健康科技能力提升项目“宏基因组二代测序技术在血液病患者感染中的应用价值”(2021LC056);吉林省科技厅创新人才(团队)项目“吉林省精准医疗多组学研究创新团队”(20220508080RC)。

摘　　要：探讨宏基因组二代测序(mNGS)在血液病合并感染诊疗中的临床应用价值。对比分析2021年8月至2022年12月吉林省人民医院血液科血液病合并感染患者的传统病原体检测结果与mNGS检测结果,探讨mNGS在血液病合并感染时诊疗的优势及临床应用价值。共纳入患者20例,经mNGS检测,16例呈阳性,包括细菌感染3例、真菌感染1例、病毒感染4例,两种以上感染8例;4例呈阴性。mNGS病原体检出率和检出种类显著高于传统检测方法。根据mNGS检测结果调整临床用药后,治疗效果良好。与传统病原体检测方法相比,mNGS对血液病合并感染具有较高的检出率,检出更全面,显著提高了患者的生存率及生活质量,对于临床用药具有指导意义。研究结果表明病原宏基因组二代测序可为该类感染提供病原学证据以辅助血液病合并感染的治疗。This paper has explored the clinical application value of metagenomic nex-tgeneration sequencing(mNGS)to the diagnosis and treatment of hematological diseases and co-infections.The detection results of traditional pathogens and mNGS detection results of patients with hematological diseases co-infection in the Department of Hematology of Jilin Provincial People’s Hospital from August 2021 to December 2022 were compared,and the advantages and clinical application value of mNGS in the diagnosis and treatment of blood diseases co-infection were discussed.A total of 20 patients were enrolled,and 16 cases were positive after mNGS testing,including 3 cases of bacterial infection,1 case of fungal infection,4 cases of viral infection,8 cases of two or more infections,and 4 cases were negative.The detection rate of mNGS pathogens(80%),and the detection types of pathogens were significantly higher than those of traditional detection methods.After adjusting the clinical medication according to the mNGS test results,the treatment effect was good.Compared with traditional pathogen detection methods,mNGS has a higher detection rate and more comprehensive detection of blood disease co-infection,which significantly improves the survival rate and quality of life of patients,and has guiding significance for clinical medication.Pathogenic metagenomic next-generation sequencing can provide etiological evidence for this type of infection to assist the treatment of co-infection of blood diseases.

关 键 词：宏基因组二代测序 血液病 感染 传统检测方法 

分 类 号：R563.1[医药卫生—呼吸系统]