不育患者精子DNA碎片指数、血清25-羟化维生素D与精液常规参数的相关性分析  

Correlation analysis of sperm DNA fragmentation index,serum 25-hydroxy-vitamin D and semen routine parameters in infertility patients

作　　者：翟倩 张颖 张建芳 田国华 ZHAI Qian;ZHANG Ying;ZHANG Jianfang;TIAN Guohua(Department of Blood testing,Xi′an Blood Center,Shaanxi Blood Center,Xi′an 710061,China)

机构地区：[1]陕西省血液中心西安市中心血站血检科,710061 [2]空军军医大学九八六医院妇产科,西安710054 [3]空军军医大学第一附属医院妇产科,西安710032

出　　处：《中国生育健康杂志》2024年第3期237-241,共5页Chinese Journal of Reproductive Health

基　　金：空军军医大学第一附属医院学科助推计划项目(XJZT21CM49)。

摘　　要：目的 探讨男性不育患者精子DNA碎片指数(DFI)、血清25-羟化维生素D [25(OH)VD]总量与精液常规参数的相关性。方法 回顾性分析2021年11月至2022年7月在空军军医大学第一附属医院生殖医学中心就诊的627名男性不育患者的检查数据,采用计算机辅助精子分析系统分析精液常规参数,手工法检测正常形态精子百分率,流式细胞仪分析精子DFI,液相色谱-串联质谱检测系统检测血清25(OH)VD总量,采用SPSS 25.0数据分析软件分析精子DFI与精子总数、浓度、前向运动率(PR)、总活动率、正常形态精子百分率的相关性,以及血清25(OH)VD总量与精子总数、浓度、PR、总活动率、正常形态精子百分率、精子DFI的相关性。结果 精子DFI与精子总数、浓度呈负相关,但其相关系数绝对值均<0.1,临床价值较小(r=-0.082、-0.094,P均<0.05),与精子PR、总活动率、正常形态精子百分率呈负相关(r=-0.519、-0.534、-0.327,P均<0.001),差异均具有统计学意义;血清25(OH)VD总量与精子总数、浓度、PR、总活动率无相关性(r=-0.009、-0.020、0.059、0.054,P>0.05),与正常形态精子百分率呈正相关(r=0.147,P<0.001),与精子DFI呈负相关(r=-0.294,P<0.001),差异均具有统计学意义。结论 精子DFI与精子PR、总活动率、正常形态精子百分率呈明显负相关,可以在一定程度上反映精子质量,精子DFI检测与精液常规参数可以互为补充,为全面、客观评估男性生育力提供有效信息;血清25(OH)VD总量与正常形态精子百分率呈正相关,与精子DFI呈负相关,可作为评估男性生育力的参考指标。Objective To investigate the correlation between sperm DNA fragmentation index(DFI),serum 25-hydroxy-vitamin D[25(OH)VD]and semen routine parameters in male infertility patients.Methods The examination data of 627 male infertility patients in the Reproductive Medicine Center of the First Affiliated Hospital of Air Force Military Medical University from November 2021 to July 2022 were retrospectively analyzed.The routine parameters of semen were analyzed by computer assisted sperm analysis system,the percentage of sperm with normal morphology was detected by manual method,the sperm DFI was analyzed by flow cytometry,and the total amount of 25(OH)VD in serum was detected by liquid chromatography-tandem mass spectrometry detection system.SPSS 25.0 software was used to analyze the correlation between sperm DFI and total sperm count,sperm concentration,percentage of forward motile sperm(PR),total sperm motility and percentage of normal morphology sperm,as well as the correlation between total amount of serum 25(OH)VD and total sperm count,concentration,PR,total sperm motility,percentage of normal morphology sperm and sperm DFI.Results Sperm DFI was negatively correlated with total sperm count and concentration(r=-0.082,-0.094,P<0.05),but r<0.1,with limited clinical value.Sperm DFI was negatively correlated with PR,total sperm motility and percentage of normal morphology sperm(r=-0.519,-0.534,-0.327,P<0.001).Serum 25(OH)VD was not correlated with total sperm count,concentration,PR and total sperm motility(r=-0.009,-0.020,0.059,0.054,P>0.05),but was positively correlated with the percentage of normal morphology sperm(r=0.147,P<0.001),and was negatively correlated with sperm DFI(r=-0.294,P<0.001).Conclusion Sperm DFI was negatively correlated with the PR,total sperm motility and percentage of normal morphology sperm,which could reflect sperm quality to a certain extent.Sperm DFI detection and semen routine parameters could complement each other,providing effective information for a comprehensive and objective assessment

关 键 词：精子DNA碎片指数 精子浓度 精子活力 精子形态 25-羟化维生素D 

分 类 号：R69[医药卫生—泌尿科学]