锑胁迫对楸树无性系叶片解剖结构的影响  

Effects of antimony stress on leaf anatomical structure of different clones of Catalpa bungei

作　　者：刘振华[1] 邹武 汪丽 林峰[1] 张鹏 钟青山 李贵[1] 陈瑞[1] 王涛 LIU Zhenhua;ZOU Wu;WANG Li;LIN Feng;ZHANG Peng;ZHONG Qingshan;LI Gui;CHEN Rui;WANG Tao(Hunan Academy of Forestry,Changsha 410004,Hunan,China;Forestry Bureau of Pingjiang County,Pingjiang 410400,Hunan,China;Hunan Forestry Resources Investigation,Monitoring and Evaluation Center,Changsha 410004,Hunan,China;Forestry Bureau of Longshan County,Longshan 416800,Hunan,China;National Botanical Garden(North Garden),Beijing 100000,China)

机构地区：[1]湖南省林业科学院,湖南长沙410004 [2]平江县林业局,湖南平江410400 [3]湖南省林业资源调查监测评价中心,湖南长沙410004 [4]龙山县林业局,湖南龙山416800 [5]国家植物园北园,北京100000

出　　处：《湖南林业科技》2024年第2期1-9,共9页Hunan Forestry Science & Technology

基　　金：长沙市杰出青年培养计划项目(kq2009087);欧洲投资银行贷款湖南森林提质增效示范项目(OT-S-KTA1)。

摘　　要：以10个楸树无性系为研究对象,采用室内盆栽的方法,共设置4种浓度的锑胁迫处理,各处理锑元素含量分别为0、600、1200、2000 mg·kg^(-1);以石蜡切片方法观测叶片的解剖结构,探讨锑胁迫对楸树叶片解剖结构的影响。结果表明:除2-8、1-1号2个楸树无性系在2000 mg·kg^(-1)锑胁迫下其叶片中锑含量均下降外,10个楸树无性系在0~2000 mg·kg^(-1)锑胁迫下其叶片中锑含量均随着锑胁迫浓度的增加而增加。在不同浓度的锑胁迫下,5-8、0号2个无性系的叶片厚度均没有显著差异,其余无性系的叶片厚度表现出不同的差异性;随着锑胁迫浓度的增加,不同楸树无性系的叶片厚度表现出不同的变化规律。随着锑胁迫浓度的增加,10个楸树无性系的叶片上表皮厚度和下表皮厚度总体呈现先升高后下降的变化规律。在不同浓度锑胁迫下,10个楸树无性系的叶片组织结构存在一定差异;在600~2000 mg·kg^(-1)锑胁迫下,各楸树无性系平均栅海比排序为5-8号的(0.99)>1号的(0.91)=2-8号的(0.91)>0号的(0.90)>5-2号的(0.89)>8402号的(0.88)=72号的(0.88)>1-1号的(0.86)=20-01号的(0.86)>63号的(0.71)。0~2000 mg·kg^(-1)锑胁迫对楸树无性系叶片结构无明显破坏,楸树对锑具有一定的耐受性。Four concentrations of antimony stress were applied to 10 clones of Catalpa bungei in indoor pot,and the contents of antimony were 0,600,1200 and 2000 mg·kg^(-1),respectively.The anatomical structure of leaves was observed by paraffin section method,and the effect of antimony stress on the anatomical structure of leaves of C.bungei was studied.The results showed that the antimony content in leaves of 10 C.bungei clones increased with the increase of antimony stress concentration under 0~2000 mg·kg^(-1) antimony stress,except 2 clones No.2-8 and No.1-1 decreased under 2000 mg·kg^(-1) antimony stress.Under different concentrations of antimony stress,the leaf thickness of 2 clones No.5-8 and No.0 showed no significant difference,and the other clones showed different changes with the increase of antimony stress concentration.As the concentration of antimony stress increases,the leaf thickness of different C.bungei clones shows different patterns of change.The thickness of upper epidermis and lower epidermis of leaves of 10 C.bungei clones presented a pattern of first increasing and then decreasing changes.Under different concentrations of antimony stress,there were some differences in leaf structure of 10 clones.In 600~2000 mg·kg^(-1) antimony stress,average C.bungei clone grid ratio of fence tissue to sponge tissue was No.5-8(0.99)>No.1(0.91)=No.2-8(0.91)>No.0(0.90)>No.5-2(0.89)>No.8402(0.88)=No.72(0.88)>No.1-1(0.86)=No.20-01(0.86)>No.63(0.71).Under 0~2000 mg·kg^(-1) antimony stress,antimony stress did not significantly damage the leaf structure of C.bungei clones,and showed a certain tolerance to antimony.

关 键 词：锑胁迫 楸树 无性系 解剖结构 

分 类 号：S792.99[农业科学—林木遗传育种]