KASP-SNP标记鉴别白菜自交系结球或非结球性状的应用  

Application of KASP-SNP markers to detecting heading or non-heading traits in Brassica inbred lines

作　　者：张菊 王丹峰 卢银[1] 徐克东 李俐俐 陈雪平[1] 赵建军[1] ZHANG Ju;WANG Danfeng;LU Yin;XU Kedong;LI Lili;CHEN Xueping;ZHAO Jianjun(State Key Laboratory of North China Crop Improvement and Regulation/International Joint Laboratory of Vegetable Molecular Breeding Technology/Ministry of Education of China-Hebei Province Joint Innovation Center for Efficient Green Vegetable Industry/Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei/College of Horticulture,Hebei Agricultural University,Baoding 071001,China;Key Laboratory of Plant Genetics and Molecular Breeding/Henan Key Laboratory of Crop Molecular Breeding and Bioreactor/School of Life Sciences and Agronomy/Zhoukou Normal University,Zhoukou 466001,China)

机构地区：[1]省部共建华北作物改良与调控国家重点实验室/蔬菜分子育种技术国际合作联合实验室/河北省绿色高效蔬菜产业省部共建协同创新中心/河北省蔬菜种质创新与利用重点实验室/河北农业大学园艺学院,河北保定071001 [2]周口师范学院植物遗传与分子育种重点实验室/河南省作物分子育种生物反应器重点实验室/周口师范学院生命科学与农学学院,河南周口466001

出　　处：《河北农业大学学报》2024年第2期47-53,64,共8页Journal of Hebei Agricultural University

基　　金：国家青年基金项目(32002062);河南省高等学校青年骨干教师培养计划(2021GGJS139)。

摘　　要：为提高传统结球型或非结球型白菜的育种效率,提高遗传分析的准确性和育种的有效性。利用SNP分子标记,选取KBr43、KBr111、KBr233和KBr258共4套引物,对206份白菜自交系,包括148份结球型白菜和58份非结球型白菜分别进行了KASP分析。KASP分析结果表明,KBr111或KBr2582套引物显示结球白菜与不结球白菜之间存在显著差异。在由3组引物KBr43、KBr111和KBr258标记的SNP位点组成的8个单倍基因型中,发现结球型白菜具有相对较高的ATG和ATC单倍基因型百分比,而非结球型白菜具有相对高的AGG或AGC单倍基因型比例。因此,我们推断,结球白菜更倾向于ATG和ATC单倍基因型,而非结球白菜更倾向于AGG和AGC单倍基因型。Leafy head was a significant agronomic trait of distinguishing heading type and non-heading type Chinese cabbage.The development of SNP molecular markers provided an auxiliary tool for leafy vegetable breeding,which could improve the efficiency of traditional heading or non-heading type Chinese cabbage,and greatly enhance the accuracy of genetic analysis and the effectiveness of breeding.In this study,206 inbred lines of Brassica accessions,including 148 heading type and 58 non-heading type Chinese cabbages,were subjected respectively to KASP analysis using four sets of primers,including KBr43,KBr111,KBr233 and KBr258.There were significant differences of the KASP results between heading and non-heading Chinese cabbage using primers KBr111 or KBr258.Among the eight haplotypes composed of SNP sites labeled with three primers sets KBr43,KBr111 and KBr258,a relatively high percentage of ATG and ATC haplotypes was found in heading type Chinese cabbage,while non-heading type Chinese cabbage harbored a relatively high percentage of AGG or AGC haplotypes.Therefore,we inferred that heading Chinese cabbage was more inclined towards ATG and ATC haplotypes,with the difference being that non-heading Chinese cabbage was more tended to AGG and AGC haplotypes.

关 键 词：蔬菜 结球或非结球农艺性状 芸薹属 KASP SNP分子标记 

分 类 号：S634.1[农业科学—蔬菜学]