河北省两类奶牛场大肠杆菌耐药性检测及多位点序列分型分析  

Detection of drug resistance of Escherichia coli in two types of dairy farms in Hebei Province and analysis of multi-locus sequence typing

作　　者：魏玉杰 李莹洁 王旭丹 梁春彩 刘聚祥[1] WEI Yujie;LI Yingjie;WANG Xudan;LIANG Chuncai;LIU Juxiang(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Hebei Veterinary Medicine and Feed Station,Shijiazhuang 050000,China;Shijiazhuang Gaocheng Animal Husbandry Work Station,Shijiazhuang 052160,China)

机构地区：[1]河北农业大学动物医学院,河北保定071000 [2]河北省兽药饲料工作总站,河北石家庄050000 [3]石家庄市藁城区畜牧工作总站,河北石家庄052160

出　　处：《河北农业大学学报》2024年第2期82-90,96,共10页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金资助项目(C2021204028);河北省现代农业产业技术体系蛋鸡产业创新团队资助(HBCT2018150210)。

摘　　要：为研究抗菌药物减量使用对细菌耐药性的影响,本试验从抗菌药物减量使用达标奶牛场和非达标奶牛场采集肛拭子样品,进行常规细菌分离鉴定,对分离株进行耐药表型、基因型检测和多位点序列分型(Multi-locus sequencetyping,MLST)。比较两类奶牛场大肠杆菌的耐药表型和基因型的差异性。结果显示,从174个肛拭子样品中分离到了173株大肠杆菌,达标场84株,非达标场89株,总体分离率为99.4%。耐药性检测发现,达标场和非达标场大肠杆菌耐药率无显著性差异(P>0.05)。分离株中共有23株大肠杆菌具有多重耐药性,多数五重及以上耐药菌株来自于非达标场。除QnrB基因达标场检出率高于非达标场外(P<0.05),两类奶牛场其他耐药基因检出率差异不显著(P>0.05)。MLST分型发现25种ST类型,其中20种为已知ST型,5种新ST型。达标场大肠杆菌的优势型为ST109型,非达标场大肠杆菌的优势型为ST10和ST1307型。奶牛场大肠杆菌的耐药表型、基因型与ST型之间无相关性。In order to study the effect of antimicrobial reduction on bacterial resistance,anal swab samples were collected from standard dairy farms and non-standard dairy farms for routine bacterial isolation and identification,whose drug resistance phenotype,genotype detection and multi-locus sequence typing(MLST)were analyzed.To compare the differences in drug resistance phenotypes and genotypes of E.coli in two types of dairy farms.The results showed that 173 strains of E.coli were isolated from 174 anal swab samples that was composed of 84 strains from the standard farm and 89 strains from the non-standard farm,and the overall isolation rate was 99.4%.The drug resistance test showed that there was no significant difference in the drug resistance rate of E.coli in the standard farm and the non-standard farm(P>0.05).A total of 23 isolates of E.coli were multi-resistant,among which most of high resistant strains(≥fivefold-resistant)came from the non-standard farms.There was no significant difference in the detection rate of other drug resistance genes between the two types of dairy farms(P>0.05),except that the detection rate of QnrB gene in the standard farm was higher than that in the non-standard farm(P<0.05).Twenty-five ST types were identified by MLST typing,in which 20 were known ST types and 5 were new ST types.ST109 was the dominant type of E.coli in the standard farm,and ST10 and ST1307 were the dominant types of E.coli in the non-standard farm.There was no correlation between the antimicrobial resistance phenotype,genotype and ST type of E.coli in dairy farms.

关 键 词：大肠杆菌 耐药性 耐药基因 多位点序列分型 

分 类 号：S852.61[农业科学—基础兽医学]