天花粉对2型糖尿病大鼠糖脂代谢和胰腺组织的影响  

Effects of Tianhuafen on glucose and lipid metabolism and pancreatic tissue in type 2 diabetic rats

作　　者：刘垚蕾 常旭 卢毅 屈阳萍 王健康 胡晓凤[1] LIU Yaolei

机构地区：[1]黄河科技学院医学院,河南郑州450000

出　　处：《中医临床研究》2024年第10期76-81,共6页Clinical Journal Of Chinese Medicine

基　　金：省级大学生创新创业训练计划一般项目(S202111834043)。

摘　　要：目的:探讨中药天花粉对高脂膳食和链脲佐菌素联合诱导的2型糖尿病大鼠的治疗作用及其可能的作用机制。方法:从60只雌雄各半的SD大鼠中随机抽取10只作为正常组,使用普通饲料喂养,剩余的50只大鼠使用高脂饲料喂养。4周后,腹腔注射链脲佐菌素(50 mg/kg),分别于注射后48 h、72 h测定大鼠空腹血糖,将连续2次空腹血糖≥16.7 mmol/L的大鼠确定为实验性糖尿病大鼠模型,根据血糖值将糖尿病大鼠随机分为模型组、二甲双胍组(0.3 mg/kg)、天花粉低剂量组(1.12 g/kg)、天花粉中剂量组(1.68 g/kg)和天花粉高剂量组(2.25 g/kg),每组10只。每天固定时间统一灌胃,持续4周,每周固定时间观察大鼠的一般症状,并测定其摄食量、摄水量、体质量和尿量。给药4周后,所有大鼠提前禁食不禁水12 h,尾静脉采血0.05 mL,测定大鼠空腹血糖,腹主动脉采血后处死大鼠并检测以下指标:各组大鼠的胰腺组织苏木精-伊红切片的病理改变,血清标本中的低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、总胆固醇、三酰甘油、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶等指标的含量。以免疫组化法检测胰腺组织中葡萄糖转运蛋白4的表达。结果:天花粉各给药组大鼠的空腹血糖浓度明显比模型组低(P<0.01或P<0.05);苏木精-伊红染色结果表明,天花粉能够在一定程度上抑制各组2型糖尿病大鼠胰腺组织的病理学改变;天花粉能够显著提升糖尿病大鼠血清中高密度脂蛋白胆固醇的含量,并降低三酰甘油、总胆固醇、低密度脂蛋白胆固醇水平(P<0.05);天花粉给药组大鼠血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶表达量明显降低(P<0.05);天花粉能上调糖尿病大鼠胰腺组织中葡萄糖转运蛋白4的水平(P<0.05)。结论:天花粉能够改善2型糖尿病大鼠的糖脂代谢,保护胰腺及重要脏器,其作用机制可能与其激活机体的葡萄糖转�Objective:To investigate the therapeutic effects of Tianhuafen(Radix Trichosanthis)on rats with type 2 diabetes mellitus induced by the combination of high-fat diet and streptozotocin and its possible mechanism of action.Methods:Ten rats were randomly selected from sixty SD rats of both sexes as the normal group and fed with normal chow,and the remaining 50 rats were fed with high-fat diet.Four weeks later,streptozotocin(50 mg/kg)was injected intraperitoneally,and the glucose of the rats was measured at 48 h and 72 h after the injection,respectively.Rats with 2 consecutive glucose≥16.7 mmol/L were identified as experimental diabetic rat model.The diabetic rats were randomly divided into the model group,the metformin group(0.3 mg/kg),the Tianhuafen lowdose group(1.12 g/kg),the Tianhuafen medium-dose group(1.68 g/kg),and the Tianhuafen high-dose group(2.25 g/kg)according to the blood glucose value,with 10 rats in each group.The rats were uniformly gavaged at a fixed time every day for 4 weeks,and the general symptoms of the rats were observed at a fixed time every week,and their food intake,water intake,body weight and urine output were measured.After 4 weeks of administration,all rats were fasted without water for 12 hours in advance,and 0.05 mL of blood was collected from the tail vein to determine the glucose of the rats,and blood was collected from the abdominal aorta,then the rats were put to death and tested for the following indexes:pathological alterations in HE sections of pancreatic tissues of the rats in each group,serum specimens of low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),total cholesterol(TC),triglyceride(TG),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)in serum specimens.glucose transporter protein 4(GLUT4)expression in pancreatic tissues was detected by immunohistochemistry.Results:The-glucose concentration of rats in each administration group of Tianhuafen was significantly lower than the model group(P<0.01 or P<0.05).HE staining r

关 键 词：天花粉 糖尿病 糖脂代谢 葡萄糖转运蛋白4 

分 类 号：R284.5[医药卫生—中药学]