荧光探针四苯基-1,3-丁二烯在四环素检测上的应用  

Application of Fluorescent Probe Tetraphenyl-1,3-Butadiene in the Detection of Antibiotics

作　　者：王瑞 郑露莹 胡博 张新宇 赵思思 张航 WANG Rui;ZHENG Lu-ying;HU Bo;ZHANG Xin-yu;ZHAO Si-si;ZHANG Hang(College of Chemistry and Chemical Engineering,Shenyang Normal University,Shenyang 110034,China;Center for Intelligent and Biomimetic Systems,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China)

机构地区：[1]沈阳师范大学化学化工学院,辽宁沈阳110034 [2]中国科学院深圳先进技术研究院智能仿生中心,广东深圳518055

出　　处：《光谱学与光谱分析》2024年第5期1321-1329,共9页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金青年科学基金项目(22109105);国家级大学生创新创业训练计划项目(202210166012);沈阳师范大学大学生创新创业训练计划项目(X202210166247)资助。

摘　　要：抗生素具有抗菌活性强、成本低等优点,已广泛应用于生产生活中。然而,抗生素的滥用可能导致其在人体内或食物中的积累,造成公共卫生问题,如引起耳毒性、肾毒性、过敏反应和细菌耐药性。因此,选取具有灵敏度高、选择性好、制备简单、快速、可用于实际样品检测等优点的荧光检测技术,对提高食品安全性和合理用药具有重要意义。基于荧光技术在检测抗生素方面的应用,选取具有AIE效应的典型代表四苯基-1,3-丁二烯(TPB),其具有化学性能稳定、发光性能好、不受溶液条件限制等优点,将其制成荧光探针用来探究TPB的光化学性质及抗生素对其猝灭效应的影响。研究结果表明,TPB的最大激发波长为365 nm,最大发射波长为435 nm,TPB固体有很强的荧光特性;在365 nm的激发波长下,TPB的量子产率为51.9%;在水体积分数为80%时,TPB的荧光效果最佳;荧光探针TPB对四环素类抗生素具有较高的选择性和较强的抗干扰能力,即四环素类抗生素对TPB高度猝灭,满足荧光内滤效应的条件,而磺胺类和喹诺酮类抗生素对TPB猝灭效果不佳;体系荧光强度随着盐酸四环素浓度增加而降低,存在较强的相关性,线性回归方程为y=-1.338x+984.20,检出限为0.0427 mmol·L^(-1);在pH≤7时,猝灭效果较好;加入盐酸四环素溶液后,湖水、海水、牛奶体系荧光强度均明显减弱,可以通过荧光强度的变化间接反映四环素类抗生素溶液浓度的变化。通过抗生素猝灭TPB的探究,在环境和食品中抗生素残留量的检测具有重大意义,为人类治理食品和环境中残留的抗生素提供参考。Antibiotics have the advantages of strong antibacterial activity and low cost and have been widely used in production and daily life.However,the abuse of antibiotics may lead to their accumulation in the human body or food,causing public health problems such as ototoxicity,nephrotoxicity,allergic reactions,and bacterial resistance.Therefore,this article selects fluorescence detection techniques with advantages such as high sensitivity,good selectivity,simple preparation,fast speed,and practical sample detection,which are significant for improving food safety and rational drug use.Based on the application of fluorescence technology in detecting antibiotics,this article selects tetraphenyl-1,3-butadiene(TPB),a typical representative with AIE effect,which has advantages such as stable chemical properties,good luminescence performance and is not limited by solution conditions.It is made into a fluorescence probe to explore the photochemical properties of TPB and the impact of antibiotics on its quenching effect.The research results show that the maximum excitation wavelength of TPB is 365 nm,and the maximum emission wavelength is 435 nm.TPB has strong fluorescence characteristics.At an excitation wavelength of 365 nm,the quantum yield of TPB is 51.9%.When the water volume fraction is 80%,the fluorescence effect of TPB is the best.The fluorescent probe TPB has high selectivity and strong anti-interference ability to tetracycline antibiotics.Tetracycline antibiotics have a high quenching effect on TPB,which meets the condition of fluorescence internal filtering effect,while sulfonamides and quinolones have a poor quenching effect on TPB.The fluorescence intensity of the system decreases with the increase of tetracycline hydrochloride concentration,and there is a strong correlation.The linear regression equation is y=-1.338x+984.20,and the detection limit is 0.0427 mmol·L^(-1);When pH≤7,the quenching effect is better;After adding tetracycline hydrochloride solution,the fluorescence intensity of the lake water,sea wat

关 键 词：荧光检测 抗生素 聚集诱导发光效应 四苯基-1 3-丁二烯 

分 类 号：O657.3[理学—分析化学]