转抗草甘膦基因甘蓝型油菜根际土壤理化性质及真菌群落多样性  

Physicochemical properties and fungal community diversity in rhizosphere soil of transgenic glyphosateresistant Brassica napus

作　　者：杜坤 李金萍[1,2] 王婷 张晶晶 王明[1,2] 王幼平 DU Kun;LI Jinping;WANG Ting;ZHANG Jingjing;WANG Ming;WANG Youping(Basic Experimental Teaching Center of Life Sciences,Yangzhou University,Yangzhou 225009,Jiangsu,China;College of Biosciences and Biotechnology,Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]扬州大学生命科学基础实验教学中心,江苏扬州225009 [2]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《生态学杂志》2024年第4期1082-1091,共10页Chinese Journal of Ecology

基　　金：转基因生物新品种培育重大专项(2018ZX08020001-012)资助。

摘　　要：在转基因作物环境安全评价研究中,对外源基因的导入是否会影响土壤微生物群体的生态平衡以及非靶标生物方面一直存在较大的争议。本研究以转抗草甘膦mEPSPS基因甘蓝型油菜JHX6(转基因油菜,GMO)和受体材料J9707(非转基因油菜,GMO-free)为对象,比较了两材料不同发育时期根际土壤理化性质,并利用IonS5TMXL的ITS高通量测序技术,对大田和盆栽试验中的根际土壤进行真菌群落特征分析。结果显示,两材料同一发育时期根际土壤理化性质均不存在显著性差异。甘蓝型油菜根际富集的主要真菌门为油壶菌门和子囊菌门,两材料在相同发育时期根际土壤中真菌的Alpha多样性指数、群落结构均没有显著差异。但是,无论盆栽还是大田试验,两材料根际土壤中油壶菌门的相对丰度均存在显著差异。因此,我们推测,在甘蓝型油菜中导入mEPSPS基因并不会显著改变根际土壤的功能、真菌群落结构和多样性,但会影响个别真菌物种的相对丰度。In the study of environmental safety assessment of transgenic crops,whether the introduction of exogenous genes will affect the ecological balance of soil microbial populations and non-target organisms has been controversial.In this study,we analyzed the differences in rhizosphere soil physicochemical properties of glyphosateresistant Brassica napus overexpressing mEPSPS(JHX6)(GMO)and the control material(J9707)(GMO-free)in different developmental stages.The fungal communities of rhizosphere soil in field and pot experiments were analyzed using ITS high-throughput sequencing technology of IonS5TMXL.Results showed that there was no significant difference in physicochemical properties of rhizosphere soil between the two materials at the same developmental stages.The main fungal phyla enriched in the rhizosphere soil of B.napus were Olpidiomycota and Ascomycota.There were no significant differences of alpha diversity and structure of fungal community in the rhizosphere soil of both GMO and GMO-free at the same developmental stages.There was a significant difference in the relative abundance of Olpidiomycota in the rhizosphere soil of the two materials in both pot and field experiments.Our results suggest that the mEPSPS introduction in B.napus did not affect rhizosphere soil physicochemical properties and fungal community structure and diversity,but would affect the relative abundance of specific fungal species.

关 键 词：mEPSPS基因 甘蓝型油菜 根际 真菌多样性 群落结构 

分 类 号：S154.3[农业科学—土壤学] S153[农业科学—农业基础科学] S565.4