UPLC-MS/MS法鉴选枣胚败育发生的关键代谢物及激素  

Identification and screening of key metabolites and hormones during Chinese jujube embryo abortion by UPLC-MS/MS

作　　者：任海燕[1] 李登科 王永康 赵爱玲[1] 薛晓芳 苏万龙 石美娟 刘丽 李毅 REN Haiyan;LI Dengke;WANG Yongkang;ZHAO Ailing;XUE Xiaofang;SU Wanlong;SHI Meijuan;LIU Li;LI Yi(Shanxi Provincial Key Laboratory of Genetic Improvement and Use of Pomology,Institute of Pomology,Shanxi Agricultural University,Taigu,Shanxi 030815,China)

机构地区：[1]山西农业大学果树研究所,果树种质创制与利用山西省重点实验室,山西太谷030815

出　　处：《植物生理学报》2024年第3期507-517,共11页Plant Physiology Journal

基　　金：山西省自然科学基金(202103021224147);山西省科技重大专项计划“揭榜挂帅”项目(202201140601027);农业农村部种质资源保护专项(111721301354052002);国家科技资源共享服务平台项目(NHGRC2023-NH12-1);山西省农业科学院科研创新团队培育专项(YGC2019TD04);现代农业产业技术体系专项(CARS-30-1-07和CARS-30-ZZ-22)。

摘　　要：为深入了解枣胚发育过程中激素和代谢物变化,揭示枣胚败育发生的生理机制。本研究以胚低败育枣品种‘灰枣’(H)和胚高败育枣品种‘孔府酥脆’(K)幼胚为试材,采用超高效液相色谱-串联质谱(UPLC-MS/MS)技术对2种材料发育不同时期胚的代谢物和激素差异进行分析。结果表明,从2个品种胚不同发育过程中共鉴定出744个代谢物,差异代谢物597个;同H品种相比,K品种在胚败育高峰期有283个差异代谢物,且与激素相关的差异代谢物主要富集在色氨酸代谢(Ko00380)、类胡萝卜素生物合成(Ko00906)、玉米素生物合成(Ko00908)、植物激素信号转导(Ko04075)、二萜类化合物生物合成(Ko00904)通路;从2个品种胚发育过程中共鉴定出27种激素,其中生长素类(ME-IAA、IAA、IAA-Glc)、细胞分裂素类(IPR、tZR、tZ、DH_(2)7G)、赤霉素类(GA_(15))、脱落酸类(ABA)与枣胚败育显著相关。本研究表明,枣胚败育可能与IPR、ABA、DHZ7G、GA15、ME-IAA含量以及酚酸类(pmb3055、Zmhn002422、Lmtn003441)、脂质类(Zmgn005057)、黄酮类(mws0789)物质合成密切相关,且相关激素和代谢物主要集中在Ko04075代谢通路上,为日后以降低枣胚败育率为目的的代谢通路靶向改造和激素调控提供依据。To reveal the changes of hormones and metabolomics during jujube(Ziziphus jujuba)embryo development and elucidate the physiological mechanism of jujube embryo abortion,the young embryos of low abortion rate jujube variety'Huizao'(H)and high abortion rate jujube variety'Kongfusucui'(K)were used as experimental materials.Ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was used to analyze the differences in metabolites and hormone components and contents of embryos at different developmental stages of the two materials.A total of 744 metabolites were identified from the different development stages of embryos of H and K varieties,and 597 differential metabolites were identified.A total of 283 differential metabolites were identified in the embryos of H and K at the peak period of embryo abortion,which were mainly enriched in tryptophan metabolism(Ko00380),carotenoid biosynthesis(Ko00906),plant hormone signal transduction(Ko04075),zeatin biosynthesis(Ko00908),diterpenoid biosynthesis(Ko00904).During the development of jujube embryos,27 related hormones were identified.There were significant correlations between embryo abortion and 9 hormones including methyl indole-3-acetate(ME-IAA),indole-3-acetic acid(IAA),1-O-indol-3-ylacetylglucose(IAAGlc),N6-isopentenyladenosine(IPR),trans-zeatin riboside(tZR),dihydrozeatin-7-glucoside(DH_(2)7G),transzeatin(tZ),gibberellin A15(GA_(15))and abscisic acid(ABA).The serious abortion of jujube embryo may be closely related to the content of IPR,ABA,DHZ7G,GA_(15),ME-IAA,as well as the synthesis of phenolic acids(pmb3055,Zmhn002422,Lmtn003441),lipids(Zmgn005057)and flavonoids(mws0789).In addition,the related hormones and metabolites are mainly concentrated in the metabolic pathway of Ko04075,which provides a scientific basis for the targeted transformation of metabolic pathways and hormone regulation for the purpose of reducing the abortion rate of jujube embryo in the future.

关 键 词：枣 胚败育 代谢物 激素 

分 类 号：S665.1[农业科学—果树学]