昆明市一起聚集性发热疫情中腺病毒3型Penton base、Hexon和Fiber基因遗传进化分析  

Genetic evolution of Penton base,Hexon and Fiber genes of human adenovirus 3 in a clustered fever outbreak in Kunming

作　　者：刘艳艳 古文鹏[2] 段忠文 王钰 龚娇 茶琦雁 吴林蔚 侯敏[1] Liu Yanyan;Gu Wenpeng;Duan Zhongwen;Wang Yu;Gong Jiao;Cha Qiyan;Wu Linwei;Hou Min(Microbiological Laboratory,Kunming Municipal Center for Disease Control and Prevention,Kunming 650228,China;Institute of Acute Infectious Diseases,Yunnan Provincial Center for Disease Control and Prevention,Kunming 650022,China;Clinical Laboratory,Chenggong District Center for Disease Control and Prevention,Kunming 650500,China;Health Monitoring and Inspection Department,Yuxi Municipal Center for Disease Control and Prevention,Yuxi 678400,China)

机构地区：[1]昆明市疾病预防控制中心微生物检验科,昆明650228 [2]云南省疾病预防控制中心急性传染病防制所,昆明650022 [3]昆明市呈贡区疾病预防控制中心检验科,昆明650500 [4]玉溪市疾病预防控制中心卫生监测检验科,玉溪678400

出　　处：《中华微生物学和免疫学杂志》2024年第3期241-248,共8页Chinese Journal of Microbiology and Immunology

基　　金：昆明市卫生科技人才培养项目暨"十百千"工程(2019-sw(后备)-51)。

摘　　要：目的对昆明市一起聚集性发热疫情样本进行腺病毒检测和遗传进化分析。方法采集典型发热患者咽拭子标本,使用微流体芯片技术进行30种常见呼吸道传染病病原体核酸检测。对腺病毒核酸阳性标本使用Nanopore三代高通量测序技术进行Penton base、Hexon和Fiber三个重要基因全长序列扩增、测定和分型鉴定,构建系统进化树,开展分子变异、遗传进化等分析。结果共采集5份标本,均检出呼吸道腺病毒及流感嗜血杆菌。对腺病毒进行Nanopore三代基因测序最终均获得Penton base、Hexon和Fiber基因全长编码区核苷酸序列,5份标本之间3个基因核苷酸相似性依次为100.0%、99.9%~100.0%和100.0%,氨基酸同源性均为100.0%;与HAdV3原型株(AY599834)核苷酸相似性最高,分别为98.6%、98.7%和98.9%。系统发育分析表明,3个基因系统进化树分析结果基本一致,人腺病毒3型(HAdV3)原型株单独一支,本研究中HAdV3序列均划分在同一进化分支上,与较早年份国外及中国台湾毒株亲缘关系较远,与近年国内外毒株亲缘关系较近,与2019年日本株(LC703523)和中国广州株(MZ540961)高度同源。与原型株比较分析昆明HAdV3氨基酸突变位点,Penton base有5处变异;Hexon有10处变异;Fiber有11处变异。结论本起疫情中的腺病毒在Penton base、Hexon和Fiber基因全长序列中型别分型结果一致,为P3H3F3型,与包括原型株在内的国内外HAdV3毒株显示出非常小的遗传变异。与原型株相比发生了多处氨基酸位点突变,并且多数氨基酸位点变异发生在蛋白质的高变区或功能区,其中Hexon蛋白中M7L为昆明序列独有氨基酸突变位点。Objective To perform adenovirus detection and genetic evolutionary analysis on specimens from a fever outbreak in Kunming city.Methods Pharyngeal swabs from typical febrile patients were collected and tested for nucleic acids of 30 common respiratory pathogens using TaqMan Array Card technology.The full-length sequences of three important genes of adenovirus,Penton base,Hexon and Fiber,were amplified,sequenced and typed using Nanopore high-throughput sequencing.A phylogenetic tree was constructed.Molecular variations and genetic evolution of the three genes were analyzed.Results Five specimens were collected and all of them tested positive for adenovirus and Haemophilus influenzae.The sequences of the full-length coding regions of the Penton base,Hexon and Fiber genes were obtained by Nanopore sequencing.The homology of the three gene sequences in the five specimens was 100.0%,99.9%-100.0%and 100.0%in nucleotide sequences,and 100.0%in amino acid sequences.The three genes in the specimens had the highest homology with those of the reference strain of human adenovirus type 3(HAdV3,accession number:AY599834)in nucleotide sequences,which was 98.6%,98.7%and 98.9%,respectively.Results of the phylogenetic analysis of the three genes were basically consistent.These Kunming strains were clustered into an independent clade with the reference HAdV3 strain and had a distant relationship with the strains isolated in foreign countries and Taiwan,China in the early years.They were closely related to the domestic and foreign strains in recent years and highly homologous to the 2019 Japanese strain(accession:LC703523)and the Guangzhou strain(accession:MZ540961).Compared with the reference strain,these Kunming strains had five amino acid variations in Penton base,10 in Hexon and 11 in Fiber.Conclusions All of the adenovirus strains isolated in this outbreak belong to P3H3F3 type based on the full-length sequences of Penton base,Hexon and Fiber genes.They share high homology with the domestic and foreign HAdV3 strains,including the

关 键 词：人腺病毒3型 五邻体基座蛋白 六邻体蛋白 纤维蛋白 遗传进化 

分 类 号：R511.8[医药卫生—内科学] R446.5[医药卫生—临床医学]