补饲不同剂量甘油对空怀期母羊养分表观消化率、瘤胃内环境、挥发性脂肪酸吸收的影响  

Effects of Supplementing Different Doses of Glycerol on Apparent Digestibility of Nutrients,Rumen Internal Environment,and Volatile Fatty Acids Absorption in Non-Pregnant Ewes

作　　者：江白慧 南颖 谢梦婷 齐行东 叶翠芳 努尔力·阿不力孜[1] 赵宗胜[1] JIANG Baihui;NAN Ying;XIE Mengting;QI Xingdong;YE Cuifang;NUERLI Abulizi;ZHAO Zongsheng(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《动物营养学报》2024年第4期2541-2550,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(3216200032);2023年兵团研究生创新项目;兵团NGHJG项目。

摘　　要：本试验旨在研究补饲不同剂量甘油对空怀期母羊养分表观消化率、瘤胃发酵参数、瘤胃上皮挥发性脂肪酸(VFA)吸收相关基因表达及瘤胃形态结构的影响。随机选取40只健康、年龄相近、体重接近的成年空怀期母羊,平均分配到对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组,每组10个重复,每个重复1只。其中,对照组饲喂基础饲粮,试验组除饲喂基础饲粮外,每日分别额外灌服1%、7%、12%的甘油(干物质占比,经估算每日每组各母羊平均干物质采食量约为1.3 kg,各组甘油灌服量分别为10、70、120 mL,甘油纯度:99.88%,燃烧能值:18.1 MJ/kg)。预试期14 d,正试期60 d。结果表明:1)试验Ⅱ组中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)、粗灰分(Ash)表观消化率显著高于对照组(P<0.05),且试验Ⅱ组NDF和ADF表观消化率也显著高于试验Ⅰ组(P<0.05),其余各组之间均无显著差异(P>0.05)。2)试验Ⅰ组瘤胃液pH显著低于对照组与试验Ⅱ组(P<0.05);与对照组相比,试验Ⅰ组和试验Ⅲ组的瘤胃内总挥发性脂肪酸(TVFA)含量显著高于对照组(P<0.01),试验Ⅱ组显著高于对照组(P<0.05);对照组瘤胃乙酸、丙酸含量与试验Ⅰ组、试验Ⅲ组差异极显著(P<0.01),与试验Ⅱ组差异显著(P<0.05);丁酸含量、各VFA占比及乙酸/丙酸在各组之间差异不显著(P>0.05)。3)随着甘油饲喂量的增加,下调式腺瘤载体(DRA)、Na^(+)/H^(+)交换蛋白3(NHE3)和质子泵型ATP酶(vH^(+)ATPase)在瘤胃上皮中的表达先增后降,而单羧酸转运蛋白1(MCT1)、单羧酸转运蛋白4(MCT4)和Na^(+)/H^(+)ATP酶(Na^(+)/K^(+)ATPase)在瘤胃上皮中的表达逐渐下降。Na^(+)/H^(+)交换蛋白1(NHE1)和Na^(+)/H^(+)交换蛋白2(NHE2)在各组之间相对表达量并无显著差异(P>0.05)。4)对照组、试验Ⅰ组与试验Ⅱ组瘤胃乳头形态良好,试验Ⅲ组瘤胃角质层出现受损现象;各试验组乳头宽度极显著低于对照组(P<0.01),乳头高度显著高于对照�This study was conducted to investigate the effects of supplementing different doses of glycerol on the apparent digestibility,rumen fermentation parameters,expression of genes related to volatile fatty acids(VFA)absorption in rumen epithelium,and rumen morphological structure in non-pregnant ewes.Forty healthy adult ewes of similar age and weight were randomly assigned to one of four groups:control,test group Ⅰ,test group Ⅱ,and test group Ⅲ,each group consisted of 10 replicates with one animal per replicate.The control group was fed a basic diet.In addition to the basic diet,the test groups were drenched daily with 1%,7%,or 12 %glycerol(dry matter basis,calculated based on an estimated daily dry matter intake of approximately 1.3 kg per ewe in each group,equating to glycerol drench volumes of 10,70 and 120 mL,respectively;purity:99.88%;calorific value:18.1 MJ/kg).A pre-feeding period of 14 days was followed by a main feeding period of 60 days.The results showed as follows:1)the neutral detergent fiber(NDF),acid detergent fiber(ADF),and ash apparent digestibility in test group Ⅱ were significantly higher than those in the control group(P<0.05),and the NDF and ADF apparent digestibility in test group Ⅱ were also significantly higher than those in test group Ⅰ(P<0.05),with no significant difference between the other groups(P>0.05).2)In test group Ⅰ,the ruminal fluid pH was significantly lower than that in the control group and test group Ⅱ(P<0.05).Compared with the control group,the total volatile fatty acids(TVFA)content in the rumen was significantly higher in test groupⅠand test group Ⅲ(P<0.01),and significantly higher in test group Ⅱ(P<0.05).The contents of acetate and propionate in the control group were significantly different from those in test group Ⅰ and test group Ⅲ(P<0.01),and significantly different from test group Ⅱ(P<0.05).The butyrate content,the proportions of various VFAs,and the acetate to propionate ratio showed no significant differences among the groups(P>0.05).3)Wit

关 键 词：甘油 空怀期母羊 瘤胃内环境 VFA 表观消化率 

分 类 号：S826[农业科学—畜牧学]