刺五加多酚合成纳米氧化锌及其抗氧化和抗菌性能研究  

Synthesis of Zinc Oxide Nanoparticles Using Polyphenols from Acanthopanax senticosus and Its Antioxidant and Antibacterial Properties

作　　者：李莹[1] 褚秀玲[1] 李晓丽 丁一 冯艺超 苏建青[1] LI Ying;CHU Xiuling;LI Xiaoli;DING Yi;FENG Yichao;SU Jianqing(Agricultural Science and Engineering School,Liaocheng University,Liaocheng 252000,China)

机构地区：[1]聊城大学农学与农业工程学院,聊城252000

出　　处：《动物营养学报》2024年第4期2648-2659,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(32172901)。

摘　　要：本试验旨在绿色合成纳米氧化锌并探究该纳米氧化锌的活性。以刺五加多酚为原料,绿色合成纳米氧化锌(ZnO NPs),通过紫外-可见分光光度计、X-射线衍射仪(XRD)、傅里叶变换红外光谱仪(FTIR)、扫描电镜(SEM)、透射电镜(TEM)、热重分析(TG)和粒径分析对所制得的样品进行表征,通过1,1-二苯基-2-三硝基苯肼(DPPH)和2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除试验评价其抗氧化性能,通过沙门氏菌、大肠杆菌和金黄色葡萄球菌抑菌试验评价其抗菌性能。结果表明:本试验成功合成ZnO NPs,其在361 nm处有吸收峰,形态为六方晶系纤锌矿结构,晶体平均粒径为77.45 nm;当ZnO NPs质量浓度达到6 g/L时,对DPPH自由基的清除率达到93.28%;当ZnO NPs质量浓度达到9 g/L时,对ABTS自由基的清除率达到73.83%;ZnO NPs对沙门氏菌、大肠杆菌的最小抑菌浓度为64 g/L,最小杀菌浓度为80 g/L,对金黄色葡萄球菌的最小抑菌浓度为80 g/L,最小杀菌浓度为88 g/L,且ZnO NPs对大肠杆菌、沙门氏菌、金黄色葡萄球菌的抑菌活性均高于氧化锌(ZnO)。本试验以刺五加多酚为原料成功合成ZnO NPs,该ZnO NPs具有良好的抗氧化和抗菌性能,为其进一步开发为饲料添加剂奠定了基础。This experiment aimed to study the green synthesis of zinc oxide nanoparticles(ZnO NPs)and explore its activity.ZnO NPs were synthesized by the green synthesis method with polyphenols from Acanthopanax senticosus as raw materials,and the samples were characterized by UV-visible spectrophotometer,X-ray diffractometer(XRD),Fourier transform infrared spectrometer(FTIR),scanning electron microscope(SEM),transmission electron microscope(TEM),thermogravimetric analysis(TG)and particle size analysis,antioxidant property was evaluated by the 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical scavenging tests,and the antibacterial property was evaluated by antibacterial tests of Salmonella,Escherichia coli and Staphylococcus aureus.The results showed that ZnO NPs were successfully synthesized in this experiment,with an absorption peak at 361 nm.Its morphology was observed as a hexagonal wurtzite structure with an average grain size of 77.45 nm.When the mass concentration of ZnO NPs was 6 g/L,the scavenging rate of DPPH radical reached 93.28%.When the mass concentration of ZnO NPs was 9 g/L,the scavenging rate of ABTS radical reached 73.83%.The minimum inhibitory concentration of ZnO NPs against Salmonella and Escherichia coli was 64 g/L,and the minimum bactericidal concentration was 80 g/L;the minimum inhibitory concentration of ZnO NPs against Staphylococcus aureus was 80 g/L,and the minimum bactericidal concentration was 88 g/L.The antibacterial activity of ZnO NPs against Escherichia coli,Salmonella and Staphylococcus aureus was higher than that of zinc oxide(ZnO).In conclusion,this experiment successfully synthesizes ZnO NPs with polyphenols from Acanthopanax senticosus as raw materials,and they have good antioxidant and antibacterial properties,which lays a foundation for their further development as feed additives.

关 键 词：纳米氧化锌 刺五加多酚 抗氧化性能 抗菌性能 

分 类 号：S816[农业科学—饲料科学]