外源褪黑素对大豆幼苗盐胁迫的缓解效应  

Effects of Exogenous Melatonin on Salt Stress Reduction in Soybean Seedlings

作　　者：郭远航 王洪博 白宝伟[6] 张磊 赵丰年[1,2] 吕东雪 贾婷 王兴鹏 GUO Yuanhang;WANG Hongbo;BAI Baowei;ZHANG Lei;ZHAO Fengnian;L Dongxue;JIA Ting;WANG Xingpeng(College of Water Hydraulic and Architectural Engineering,Tarim University,Alear 843300,China;Modern Agricultural Engineering Key Laboratory at Universities of Education Department of Xinjiang Uygur Autonomous Region,Tarim University,Alaer 843300,China;Key Laboratory of Tarim Oasis Agriculture,Ministry of Education,Tarim University,Alear 843300,China;Western Agricultural Research Center,Chinese Academy of Agricultural Sciences,Changji 831100,Xinjiang,China;Key Laboratory of Northwest Oasis Water-Saving Agriculture,Ministry of Agriculture and Rural Affairs,Shihezi 832000,China;College of Life Sciences,Tarim University,Alear 843300,China)

机构地区：[1]塔里木大学水利与建筑工程学院,新疆阿拉尔843300 [2]塔里木大学自治区教育厅普通高等学校现代农业重点实验室,新疆阿拉尔843300 [3]塔里木大学塔里木绿洲农业教育部重点实验室,新疆阿拉尔843300 [4]中国农业科学院西部农业研究中心,新疆昌吉831100 [5]农业农村部西北绿洲节水农业重点实验室,新疆石河子832000 [6]塔里木大学生命科学与技术学院,新疆阿拉尔843300

出　　处：《华北农学报》2024年第2期116-125,共10页Acta Agriculturae Boreali-Sinica

基　　金：新疆生产建设兵团重大科技项目(2021AA003);塔里木大学校长基金(TDZKYS202301);塔里木大学校级研究生科研创新项目(TDGRI202254)。

摘　　要：为探明外源褪黑素(MT)对大豆幼苗耐盐性的调节作用,筛选出不同盐胁迫下适宜的施用浓度。以大豆品种田友-2986为试验材料,设置3个盐浓度(低盐S_(3):3 g/L、中盐S_(5):5 g/L、高盐S_(7):7 g/L)和6个MT浓度(M_(0):0μmol/L、M_(1):25μmol/L、M_(2):50μmol/L、M_(3):75μmol/L、M_(4):100μmol/L、M_(5):150μmol/L),分析了大豆幼苗形态参数、生物量、抗氧化酶活性及渗透调节物质含量。随着盐胁迫程度的增大,大豆幼苗的根系形态参数、生物量、根冠比、抗氧化酶活性和渗透调节物质含量降低,丙二醛含量增加。低盐(S3)和中盐(S5)胁迫下,外施50μmol/L MT大豆根系总长度、侧根数、SOD和POD较不施加分别提高了52.30%,19.98%,74.10%,40.03%(低盐)和68.52%,19.24%,81.72%,37.42%(中盐);高盐(S7)胁迫下,外施75μmol/L MT分别提高了71.17%,19.11%,80.79%和27.01%。盐胁迫下,外源25~100μmol/L的MT不同程度上促进了大豆幼苗生长,提高耐盐性。隶属函数综合评价表明,在低、中盐胁迫下,50μmol/L MT的缓解盐害效果最好;高盐胁迫下,MT这一适宜浓度在75μmol/L。MT缓解大豆幼苗盐害主要在于它提高抗氧化酶活性和渗透调节物质含量,降低了丙二醛含量,从而缓解了盐胁迫下大豆的氧化胁迫和渗透胁迫。To investigate the effect of exogenous melatonin(MT)on salt tolerance of soybean seedlings,and to screen the appropriate application concentration under different salt stress.The soybean variety Tianyou-2986 was used as the test material,and 3 salt concentrations(low salt S_(3):3 g/L,medium salt S_(5):5 g/L,high salt S_(7):7 g/L)and 6 MT concentrations were set(M_(0):0μmol/L,M_(1):25μmol/L,M_(2):50μmol/L,M_(3):75μmol/L,M_(4):100μmol/L,M 5:150μmol/L),the morphological parameters,biomass,antioxidant enzyme activity and osmoregulatory substance content of soybean seedlings were analyzed.With the increase of salt stress,the root morphological parameters,biomass,root-shoot ratio,antioxidant enzyme activity and osmoregulatory substance content of soybean seedlings decreased,while the malondialdehyde content increased.Under low salt(S 3)and medium salt(S 5)stress,The number of total length,lateral roots,SOD and POD of 50μmol/L MT were increased by 52.30%,19.98%,74.10%,40.03%(low salt)and 68.52%,19.24%,81.72%and 37.42%(medium salt),respectively.Under high stress(S 7),75μmol/L MT increased by 71.17%,19.11%,80.79%and 27.01%,respectively.Under salt stress,exogenous 25-100μmol/L MT promoted soybean seedling growth and improved salt tolerance to different degrees.The overall evaluation of the affiliation function showed that 50μmol/L MT was the most effective in alleviating salt damage under low and medium salt stress,and the suitable concentration of MT was 75μmol/L under high salt stress,and the main reason for the alleviation of salt damage in soybean seedlings was that MT increased the activities of antioxidant enzymes and osmoregulatory substance content,and reduced the content of malondialdehyde,which could alleviate oxidative and osmotic stresses of soybeans under salt stress.

关 键 词：大豆 盐胁迫 褪黑素 抗氧化系统 综合评价 

分 类 号：S565.1[农业科学—作物学]