文山高峰牛朊蛋白基因(PRNP)23 bp和12 bp插入/缺失多态性分析研究  

Analysis of Wenshan Cattle Prion Protein Gene ( PRNP )23 bp and 12 bp Insertion/deletion Polymorphisms

作　　者：种玉晴 和晓明 邓文宝 农胜虎 方云霞 梁昌兴 何祖春 刘萍丹 岳丹 邓卫东[1] 席冬梅[1] CHONG Yuqing;HE Xiaoming;DENG Wenbao;NONG Shenghu;FANG Yunxia;LIANG Changxin;HE Zuchun;LIU Pingdan;YUE Dan;DENG Weidong;XI Dongmei(Faculty of Animal Science and Technology,Yunan Agricultural University,Kunming,Yunan 650201,China;Bureau of Agriculture,Rural Affairs and Science and Technology,Wenshan,Yunnan 663300,China;Yuxi Agricultural Vocational and Technical College,Yuxi,Yunnan 653106,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省广南县农业农村和科学技术局,云南文山663300 [3]玉溪农业职业技术学院,云南玉溪653106

出　　处：《家畜生态学报》2024年第4期15-21,共7页Journal of Domestic Animal Ecology

基　　金：云南省重大科技专项(202102AE090019)。

摘　　要：为探讨文山高峰牛朊蛋白基因(prion protein gene,PRNP)启动子区23 bp和第一内含子区12 bp插入/缺失多态性特征,本研究采用聚合酶链式反应(polymerase chain reaction,PCR)和琼脂糖凝胶电泳的方法对文山高峰牛PRNP基因23 bp和12 bp位点进行基因分型,得出其多态性数据;通过与文献所报道的牛PRNP数据进行比较分析,得出文山高峰牛对疯牛病抗病性的强弱。结果表明:文山牛PRNP基因启动子区23 bp等位基因频率以缺失型为主(0.654);第一内含子区12 bp等位基因频率分别为插入型0.913,缺失型0.087;缺失型单倍型频率极低(0.082);与德国、英国以及瑞士3个国家所报道的患BSE病牛和健康牛相比较存在显著差异(P<0.05),12 bp等位基因插入/缺失频率与水牛不存在显著差异(P>0.05)。由此可见,较高的12 bp插入型等位基因和极低的缺失型单倍(23 bp插入-12 bp缺失)频率,使得文山高峰牛对BSE疾病具有较好抗病力,通过抗病育种分析能够有效预防疯牛病的发生;对中国地方牛种质资源进行相关抗病性评估,有利于为牛的抗病分子选育提供数据支撑。To investigate the polymorphisms of the promoter region 23 bp and the first intron region 12 bp insertion/deletion of Wenshan cattle prion protein gene(PRNP),the polymorphism data of PRNP gene 23 bp and 12 bp of Wenshan cattle were genotyped by polymerase chain reaction and agarose gel electrophoresis.Through comparative analysis with the PRNP data of cattle reported in the literature,the resistance of Wenshan cattle to mad cow disease was obtained.Results showed the frequency of the 23 bp allele in the promoter region of the PRNP gene of Wenshan cattle is predominantly represented by the deletion type(0.654).The frequencies of the 12 bp insertion and deletion alleles in the first intron region are 0.913 and 0.087,respectively,and the frequency of the deletion haplotype is very low(0.082).A significant difference(P<0.05)was observed when comparing the frequencies of the 12 bp insertion/deletion alleles in Wenshan cattle with those reported in cases of BSE-affected and healthy cattle from Germany,the United Kingdom,and Switzerland.However,no significant difference(P>0.05)was found in the insertion/deletion allele frequencies between Wenshan cattle and water buffalo.Therefore,it is concluded that the relatively high frequency of the 12 bp insertion allele and the extremely low frequency of the deletion type haplotype(23 bp insertion-12 bp deletion)in Wenshan cattle confer a higher resistance to BSE disease,making them a valuable resource for disease-resistant breeding programs.Analysis of disease resistance in local cattle breeds in China is beneficial for providing data support for molecular breeding of disease resistance in cattle breeds.

关 键 词：文山高峰牛 朊蛋白基因(PRNP) 疯牛病(BSE) 多态性 

分 类 号：S823.2[农业科学—畜牧学]