大鼠周围神经损伤后外周血内皮祖细胞动员及相关因子含量变化  

Changes in peripheral blood endothelial progenitor cell mobilization and related factors after peripheral nerve injury in rats

作　　者：赵斌[1] 赵志虎[1] 骆巍 马剑雄[1] 马信龙[1] ZHAO Bin;ZHAO Zhihu;LUO Wei;MA Jianxiong;MA Xinlong(Digital Orthopedic Technology Clinical Application Center,Tianjin Hospital,Tianjin 300211,China)

机构地区：[1]天津市天津医院数字骨科技术临床应用中心,300211

出　　处：《天津医药》2024年第5期459-462,共4页Tianjin Medical Journal

基　　金：国家自然科学基金青年基金项目(81501061);天津市卫生健康行业高层次人才选拔培养工程-青年医学新锐(TJSQNYXXRD2-136);天津市卫生健康科研项目(TJWJ2023QN049)。

摘　　要：目的探讨大鼠周围神经损伤(PNI)后外周血内皮祖细胞(EPCs)、碱性成纤维细胞生长因子(bFGF)、血管内皮生长因子(VEGF)及基质金属蛋白酶-9(MMP-9)水平变化及EPCs与其他指标的相关性。方法42只SD大鼠按随机数字表法分为对照组、PNI 1 d组、PNI 3 d组、PNI 5 d组、PNI 7 d组及PNI 14 d组,每组7只,除对照组外其余组均采用钳夹法建立坐骨神经损伤模型。对每组在预定时间点采用活体心脏穿刺法采血;采用Ficoll密度梯度离心法提取单个核细胞,CD34和CD133双阳性细胞标记EPCs,应用流式细胞仪检测各组EPCs数量。采用酶联免疫吸附试验检测各组外周血bFGF、VEGF及MMP-9含量,分析EPCs数量与bFGF、VEGF、MMP-9水平的相关性。结果与对照组相比,PNI 3 d组、PNI 5 d组、PNI 7 d组外周血EPCs数量升高,PNI 3 d组、PNI 5 d组、PNI 7 d组及PNI 14 d组外周血bFGF含量升高,其余各组外周血VEGF含量升高,PNI 5 d组、PNI 7 d组及PNI 14 d组外周血MMP-9含量升高(P<0.05)。PNI 5 d组和PNI 7 d组外周血EPCs数量与血清bFGF水平呈正相关(r分别为0.784和0.788,P<0.05),与血清VEGF水平呈正相关(r分别为0.889和0.852,P<0.05);PNI 5 d组、PNI 7 d组和PNI 14 d组外周血EPCs数量与血清MMP-9水平呈正相关(r分别为0.788、0.852和0.873,P<0.05)。结论EPCs与bFGF、VEGF和MMP-9共同参与了PNI后血供修复的病理生理过程。Objective To study changes and correlation of peripheral bold endothelial progenitor cells(EPCs)and serum bFGF,VEGF and MMP-9 in rats with peripheral nerve injury(PNI).Methods Forty-two SD rats were divided into the control group,the PNI 1 d group,the PNI 3 d group,the PNI 5 d group,the PNI 7 d group and the PNI 14 d group according to random number table method,with 7 rats in each group.The sciatic nerve injury model was established in all groups except the control group.In each group,blood samples were collected by living heart puncture at a predetermined time point.Mononuclear cells were extracted by Ficoll density gradient centrifugation.CD34 and CD133 double positive cells were labeled with EPCs,and the number of EPCs in each group was detected by flow cytometry.The contents of bFGF,VEGF and MMP-9 in peripheral blood of each group were detected by enzyme-linked immunosorbent assay,and the correlation between the number of EPCs and bFGF,VEGF and MMP-9 was analyzed.Results Compared with the control group,EPCs in peripheral blood were increased in the PNI 3 d group,the PNI 5 d group and PNI 7 d group.bFGF contents in peripheral blood were increased in the PNI 3 d group,the PNI 5 d group,the PNI 7 d group and the PNI 14 d group,and VEGF contents in peripheral blood were increased in the other groups.The content of MMP-9 in peripheral blood was increased in the PNI 5 d group,the PNI 7 d group and the PNI 14 d group(P<0.05).The number of EPCs in peripheral blood in the PNI 5 d group and the PNI 7 d group was positively correlated with serum bFGF level(r=0.784 and 0.788,P<0.05)and serum VEGF level(r=0.889 and 0.852,P<0.05).The number of EPCs in peripheral blood of the PNI 5 d group,the PNI 7 d group and the PNI 14 d group was positively correlated with serum MMP-9 level(r=0.788,0.852 and 0.873,P<0.05),and it was positively correlated with serum VEGF level(r=0.889 and 0.852,P<0.05).The number of EPCs in peripheral blood was positively correlated with serum MMP-9 level in the PNI 5 d group,the PNI 7 d group and the P

关 键 词：周围神经损伤 坐骨神经 内皮祖细胞 基质金属蛋白酶9 血管内皮生长因子 碱性成纤维细胞生长因子 

分 类 号：R651.3[医药卫生—外科学]