2019-2022年宁夏回族自治区人间布鲁氏菌分离株种型鉴定及分子特征分析  

Species identification and molecular characterization of human Brucella strains isolated in Ningxia Hui Autonomous Region,2019−2022

作　　者：高磊 高建炜[3] 杨聪[3] 陈倩 冼然 赵瑜 马江涛[3] Gao Lei;Gao Jianwei;Yang Cong;Chen Qian;Xian Ran;Zhao Yu;Ma Jiangtao(School of Public Health,Ningxia Medical University,Yinchuan 750004,Ningxia,China;Key Laboratory of Environmental Factors and Chronic Disease Control,Yinchuan 750004,Ningxia,China;Ningxia Hui Autonomous Region Centre for Disease Control and Prevention,Yinchuan 750004,Ningxia,China)

机构地区：[1]宁夏医科大学公共卫生学院,宁夏银川750004 [2]宁夏环境因素与慢性病控制重点实验室,宁夏银川750004 [3]宁夏回族自治区疾病预防控制中心,宁夏银川750004

出　　处：《疾病监测》2024年第3期279-284,共6页Disease Surveillance

基　　金：宁夏自然科学基金(No.2022AAC03712);宁夏自然科学基金重点项目(No.2023AAC02075)。

摘　　要：目的 对2019-2022年宁夏回族自治区(宁夏)人间布鲁氏菌分离株进行种型和分子分型研究,掌握其分子遗传特征,为宁夏人间布鲁氏菌病(布病)的精准防控提供科学依据。方法 采用AMOS聚合酶链式反应(AMOS-PCR)对布鲁氏菌分离株进行种的鉴定,运用多位点可变数目串联重复序列分析(MLVA)对分离菌株进行分子分型,利用BioNumerics 7.6软件对MLVA结果进行聚类分析。结果 72株布鲁氏菌分离株经AMOS-PCR鉴定显示均为羊种布鲁氏菌。经MLVA分型,Panel1显示具有42、45、63共3个MLVA-8型。Panel2A显示均为41型(4-20-8),Panel2B显示具有高度多变性;72株菌被分为51个MLVA-16基因型,12个为共享基因型,其余39个为单一基因型。宁夏原州区的布鲁氏菌型别较为丰富,包含3种MLVA-8型别。本研究中的72株布鲁氏菌与多个省份的菌株存在18种完全相同的MLVA-16基因型,表明菌株不仅在宁夏广泛传播,在全国范围内也呈跨地区传播的特点。结论 宁夏人间布病疫情极为严重,通过MLVA-16确定了宁夏布鲁氏菌分离株存在多种基因型,可为后续人间布病精准防控及流行病学溯源提供科学依据。Objective To understand the species type and molecular characteristics of human Brucella isolates in Ningxia Hui Autonomous Region(Ningxia)from 2019 to 2022 and provide evidence for the precise prevention and control of human brucellosis.Methods Brucella isolates were identified by AMOS polymerase chain reaction(AMOS-PCR),molecular typing of the isolates was performed by multilocus variable number of tandem repeat sequence analysis(MLVA),and the clustering analysis was conducted by using software BioNumerics 7.6.Results A total of 72 Brucella isolates were identified by AMOS-PCR.The results showed that all the isolates were Brucella melitensis.After MLVA typing,the isolates belonged to 3 MLVA-8 types,42,45 and 63 in Panel1.The isolates all belonged to type 41(4-20-8)in Panel2A and the isolates showed a high degree of variability in Panel2B.72 isolates were classified into 51 MLVA-16 genotypes,including 12 common genotype and 39 unique genotypes.More Brucella types were found in Yuanzhou district,including three MLVA-8 types.The 72 Brucella strains in this study shared 18 identical MLVA-16 genotypes with the strains isolated in several provinces,indicating the cross-regional transmission in the country.Conclusion Human brucellosis epidemic is extremely serious in Ningxia,and MLVA-16 have found the existence of multiple genotypes of Brucella in Ningxia,providing evidence for the precise prevention and control of human brucellosis and epidemiological tracing of human brucellosis cases.

关 键 词：布鲁氏菌病 AMOS聚合酶链式反应 多位点可变数目串联重复序列分析 羊种布鲁氏菌 

分 类 号：R211[医药卫生—中医学] R516.7