靶向细胞器的过氧亚硝酸盐荧光探针研究进展  

Research Progress on Fluorescent Probes of Peroxynitrite for Targeting Organelles

作　　者：潘淑芬 马晓 蓝庆春 吴勇权 PAN Shu-fen;MA Xiao;LAN Qing-chun;WU Yong-quan(College of Chemistry and Chemical Engineering,Gannan Normal University,Ganzhou 341000)

机构地区：[1]赣南师范大学化学化工学院,江西赣州341000

出　　处：《化学试剂》2024年第5期10-19,共10页Chemical Reagents

基　　金：国家自然科学基金项目(21967001);江西省“双千计划”人才项目(jxsq2019201090);江西省自然科学基金项目(20224BAB203004)。

摘　　要：过氧亚硝酸盐(ONOO^(-))作为氧代谢的重要产物,在免疫应答、细胞信号转导等许多生理活动中起着重要作用。然而,ONOO^(-)的水平异常会损伤细胞生物分子,与许多疾病密切相关。因此,迫切需要监测活细胞中ONOO^(-)的含量变化,而ONOO^(-)的功能很大程度上依赖于亚细胞区,所以开发亚细胞的ONOO^(-)水平变化的监测手段更有研究价值。为此,多种具有靶向细胞器的ONOO^(-)荧光探针被设计、开发和应用。重点介绍了近6年来具有线粒体、溶酶体和内质网靶向能力的ONOO^(-)荧光探针的代表性例子。综述了这些荧光探针的细胞器靶向策略、结构、荧光行为和生物学应用。As an essential product of oxygen metabolism,peroxynitrite(ONOO^(-))plays an important role in various biological processes,including immune response and cell signal transduction.However,excessive ONOO^(-)production can lead to cellular biomolecule damage and is closely associated with numerous diseases.Therefore,there is an urgent need to monitor the level changes of ONOO^(-)in living cells.Given that the function of ONOO^(-)largely depends on subcellular compartments,it is particularly significant to develop probes capable of monitoring subcellular ONOO^(-)levels.To this end,a variety of ONOO^(-)fluorescent probes,specifically targeting organelles,have been designed,developed,and successfully employed.In this review,we highlighted representative cases of ONOO^(-)fluorescent probes with targeted capabilities for mitochondrial,lysosome,and endoplasmic reticulum(ER)from the last six years.The organelle-targeting strategy,structure,fluorescence behavior,and biological application of these probes were reviewed as well.

关 键 词：过氧亚硝酸盐 亚细胞水平 荧光探针 荧光检测 荧光成像 

分 类 号：O65[理学—分析化学]