不同烟草种质感染番茄斑驳花叶病毒的细胞病理特征  

Cytopathological changes of different tobacco varieties infected with tomato mottle mosaic virus

作　　者：李月月 张水英[2,4] 周文鹏[2] 张天毅 兰平秀 谭冠林 李凡 LI Yueyue;ZHANG Shuiying;ZHOU Wenpeng;ZHANG Tianyi;LAN Pingxiu;TAN Guanlin;LI Fan(College of Life Science,Luoyang Normal University,Luoyang 471934,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Center for Modern Educational Technology,Yun-nan Agricultural University,Kunming 650201,China;Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming 650201,China)

机构地区：[1]洛阳师范学院生命科学学院,洛阳471934 [2]云南农业大学植物保护学院,昆明650201 [3]云南农业大学现代教育技术中心,昆明650201 [4]云南省农业科学院生物技术与种质资源研究所,昆明650201

出　　处：《植物病理学报》2024年第1期92-101,共10页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31660039),河南省科技攻关(232102111082),洛阳师范学院国家级项目培育基金(2019-PYJJ-009),云南省魏太云专家工作站(202005AF150040)。

摘　　要：番茄斑驳花叶病毒(tomato mottle mosaic virus,ToMMV)比同属烟草花叶病毒(tobacco mosaic virus,TMV)和番茄花叶病毒(tomato mosaic virus,ToMV)在茄科作物上的侵染性更强,为明确受ToMMV侵染后烟草不同表现症状对应的细胞病理学特征。本文构建了ToMMV的侵染性克隆载体,分别对感染ToMMV的不同烟草组织中的病毒粒子及不同表现症状的烟草细胞超微结构进行了观察分析。结果表明ToMMV为长度约220~310 nm,直径约16~18 nm的杆状粒子,感染ToMMV的珊西烟(Nicotiana tabacum var.Xanthi nc)种子也可携带病毒。ToMMV病毒粒子以分散或整齐分层排列于细胞质或液泡内。感染ToMMV的珊西烟和黄花烟(N.rustica)细胞均产生多泡体结构、大量次级小囊泡以及髓鞘样结构,线粒体嵴减少或消失。其中,症状表现为花叶、畸形的珊西烟叶片细胞中叶绿体变化较大,有膜破碎、大量液泡进入的现象;线形叶细胞中多泡体结构和次级小囊泡数量更多;而表现为系统性坏死的黄花烟叶片细胞中叶绿体变化较小。本研究ToM-MV侵染性克隆的构建和不同烟草被感染后的细胞病变观察为进一步研究ToMMV的致病机理以及与寄主的互作等提供了重要条件和依据。Tomato mottle mosaic virus(ToMMV),a member of the genus Tobamovirus,showed stronger pathogenicity than tobacco mosaic virus(TMV)and tomato mosaic virus(ToMV)in most solanaceae crops.To figure out the cytopathological changes of tobacco plants that were infected by ToMMV,we constructed a full-length infectious cDNA clone of ToMMV,and carried out transmission electron microscopy observation.The results showed that the rod-shaped virions of ToMMV were 220-310 nm in length and 16-18 nm in diameter.ToMMV virions were also observed in the seeds of ToMMV-infected Nicotiana tabacum var.Xanthi nc.ToM-MV virions were dispersed or angularly-layered in cytoplasm or vacuoles.Multivesicular bodies,small vesicles,myeloid bodies,mitochondrial cristae were observed to be reduced or disappeared in both N.tabacum var.Xanthi nc and N.rustica infected with ToMMV.In addition,broken chloroplast membranes and large amount of small peripheral vesicles protruding into the disintegrated chloroplasts were observed in the infected N.tabacum var.Xanthi nc showing mosaic and distortion symptoms,while large amount of small vesicles were observed in the cells of infected N.tabacum var.Xanthi nc showing leaf narrowing.However,chloroplasts changes were less frequent in N.rustica with systemic necrosis symptoms.In this study,the construction of ToMMV infectious clone and the observation of cytopathological changes in tobacco plants infected with ToMMV,provided impor-tant bases for the further study of the pathogenesis of ToMMV and its interaction with the host.

关 键 词：番茄斑驳花叶病毒 侵染性克隆载体 细胞病变 珊西烟 黄花烟 

分 类 号：S432.41[农业科学—植物病理学]