不同发病级别小麦黄花叶病病毒含量的分析  

Study on virus content of wheat yellow mosaic disease at different disease grade

作　　者：杜梦园 刘迪 陈莉 侯艳红 陈琦 范志业 李雷雷 沈海龙 王文豪 师兴凯 李世民 DU Mengyuan;LIU Di;CHEN Li;HOU Yanhong;CHEN Qi;FAN Zhiye;LI Leilei;SHEN Hailong;WANG Wenhao;SHI Xingkai;LI Shimin(Luohe Academy of Agricultural Sciences,National Agricultural Observing and Ex-perimental Station for Plant Protection in YanCheng,Luohe 462300,China)

机构地区：[1]漯河市农业科学院,国家农业科学植物保护郾城观测实验站,漯河462300

出　　处：《植物病理学报》2024年第1期188-194,共7页Acta Phytopathologica Sinica

基　　金：国家小麦产业技术体系(CARS-03);国家科技支撑计划项目“黄淮区小麦抗逆增产技术研究集成与示范”(2015BAD26B01);河南省现代农业产业技术体系漯河综合试验站(HARS-22-01-Z4)。

摘　　要：为明确小麦黄花叶病毒(wheat yellow mosaic virus,WYMV)在不同发病级别、不同组织部位含量的差异,从小麦黄花叶病发病的田块,选取发病典型的级别为0、1、2和3级的小麦样本;取无病毒田块的健康小麦样本为空白对照,利用实时荧光定量PCR(real-time fluorescent quantitative PCR,RT-qPCR)技术,分别对小麦的根、茎、叶进行小麦黄花叶病毒含量的绝对定量。结果表明,在感病样品中,小麦根部的WYMV含量都显著多于叶部(P<0.05),发病0级小麦根部的病毒含量是叶部病毒含量的13.97倍。在不同发病级别的小麦组织中,随着发病级别的加重,根、茎、叶中的病毒含量都是显著增加的(P<0.05)。根中的病毒含量先是快速增加而后缓慢增加,0级到1级,根中病毒量增加3.95×10^(6)copies·μL^(-1),而1级到3级,病毒增加量仅为1.45×10^(6)copies·μL^(-1);茎和叶中病毒含量呈现匀速增加的趋势,且茎中病毒含量与发病级别呈强正相关性(R^(2)=0.9967)。本研究明确了感病小麦根部WYMV的含量最多,同时发现在尚未显症的发病0级小麦中,根部WYMV含量远大于对照组;茎部WYMV的含量与症状关系最为密切,茎部病毒含量越高,小麦显症越明显。In order to clarify the variation of virus content of wheat yellow mosaic virus(WYMV)in various disease grade and tissue parts,wheat samples with typical incidence levels of 0,1,2 and 3 were collected from fields with uniform incidence of wheat yellow mosaic disease.Healthy wheat samples from virus-free fields were used as blank control.The absolute quantification of the content of wheat yellow mosaic virus in roots,stems and leaves was carried out by real-time fluorescent quantitative PCR(RT-qPCR).The results showed that the content of WYMV in wheat roots was significantly higher than that in leaves(P<0.05),and the virus content in wheat roots of grade 0 was 13.97 times higher than that in leaves.In wheat tissues of different disease grade,the virus content in roots,stems and leaves increased significantly with the aggravation of disease grade(P<0.05).The virus content in roots increased rapidly and then slowed down.From grade 0 to grade 1,the virus content in roots increased by 3.95×10^(6) copies·μL^(-1)L.However,from grade 1 to grade 3,the virus increase was only 1.45×10^(6) copies·μL^(-1).The virus content in stems and leaves showed a trend of uniform increase,and there was a strong positive correlation between the virus content in stems and the disease grade(R^(2)=0.9967).This study confirmed that the root is the key tissue site of WYMV virus infection in wheat.At the same time,it was found that the content of WYMV in the roots of diseased grade 0 wheat was much higher than that in the con-trol group.The content of WYMV in the stem correlates closely with viral symptoms.The higher the content of stem virus,the more obvious the symptom of wheat.

关 键 词：小麦 小麦黄花叶病毒 绝对定量 发病级别 

分 类 号：S432.1[农业科学—植物病理学]