湖北十堰八角莲叶斑病病原菌分离及鉴定  

Isolation and identification of pathogen causing leaf spot of Dysosma versipellis in Shiyan of Hubei Province

作　　者：蔡高磊 周炀 张泽志 宋伟 张含 孙良学 柯尊伟 刘新刚[4] CAI Gaolei;ZHOU Yang;ZHANG Zezhi;SONG Wei;ZHANG Han;SUN Liangxue;KE Zunwei;LIU Xingang(Danjiangkou Reservoir of Shiyan Ecological Agriculture Institute,Shiyan 442000,China;Longwang Zhai Rare Chinese Herbal Medicine Farming Farmer Cooperatives,Shiyan 442000,China;College of Chemistry and Environmental Engineering,Hanjiang Normal University,Shiyan 442000,China;Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193,China)

机构地区：[1]丹江口库区十堰生态农业研究院,十堰442000 [2]十堰市龙王寨珍稀中草药种养农民专业合作社,十堰442000 [3]汉江师范学院化学与环境工程学院,十堰442000 [4]中国农业科学院植物保护研究所,北京100193

出　　处：《植物病理学报》2024年第1期199-203,共5页Acta Phytopathologica Sinica

基　　金：中国农业科学院科技创新工程协同创新任务(CAAS-XTCX2016015);国家重点研发计划(2019YFD1002000);湖北省高校优秀中青年科技创新团队(T2021029)。

摘　　要：八角莲[Dysosma versipellis(Hance)M.Cheng ex Ying]是小檗科(Berberidaceae)鬼臼属(Dysosma)多年生草本植物,叶片和根茎的茎残基均呈碗口状并且大多数生长在高山荫湿林地靠近水源处,其形态特征、中药化学成分和生物活性与六角莲[Dysosma pleiantha(Hance)Woodson]、南方山荷叶(Diphylleia sinensis H.L.Li)相近。八角莲根茎主要含有木脂素类、黄酮类、酚类和蒽醌类,此外还有皂苷、鞣质和多糖等化学组分[1-3],因其具有祛风除湿、清热解毒、活血散瘀、消炎止痛、镇咳平喘、解毒等功效[4],常用于治疗气管炎、外伤性肿胀、风湿腰腿疼痛、月经不调等疾病[5]。研究表明,木脂素类代表性成分鬼臼毒素还具有抗肿瘤、抗病毒、杀害虫、抗菌、抗氧化、增强免疫力等多种药理活性[6-12]。In May of 2021,a leaf spot disease of the herbal medicine Dysosma versipellis was observed,and it occured seriously in Shiyan of Hubei Province.Diseased leaves of D.versipellis were collected,pathogen was isolated by tissue cultural-method,and its pathogenicity was confirmed according to the Koch's postulates.Based on the morphological characteristics and phylogenetic analysis by combination of ITS region and EF1-αgene sequences,pathogen was identified as Alternaria alternata.This is the first report leaf spot of Dysosma versipel-lis caused by A.alternata in China.

关 键 词：祛风除湿 多年生草本植物 八角莲 活血散瘀 鬼臼毒素 镇咳平喘 腰腿疼痛 六角莲 

分 类 号：S432.44[农业科学—植物病理学]