快速高效鉴定花生青枯病抗性的水培接种新方法研究  

Development of a water culture inoculation method for quick identification of bacte-rial wilt resistance in peanut

作　　者：宋万朵 晏立英[1] 于东洋 康彦平[1] 雷永[1] 陈玉宁[1] 淮东欣[1] 王欣 王志慧[1] 罗怀勇[1] 姜慧芳[1] 廖伯寿[1] SONG Wanduo;YAN Liying;YU Dongyang;KANG Yanping;LEI Yong;CHEN Yuning;HUAI Dongxin;WANG Xin;WANG Zhihui;LUO Huaiyong;JIANG Huifang;LIAO Boshou(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan,430062,China)

机构地区：[1]油料作物生物学与遗传育种重点实验室/中国农业科学院油料作物研究所,武汉430062

出　　处：《植物病理学报》2024年第1期204-208,共5页Acta Phytopathologica Sinica

基　　金：中央级科研院所基本科研业务费专项(1610172021003)、财政部和农业农村部:国家现代农业产业技术体系(CARS-13)、国家自然科学基金(32161143006)、湖北省重点研发计划项目(2021BBA077)。

摘　　要：0引言花生(Arachis hypogaea L)是我国重要的油料和经济作物之一,受青枯病危害较重,该病害由雷尔氏青枯菌(Ralstonia solanacearum)引起,在我国主要产区均有发生,危害面积约占种植面积的1/10,居世界首位[1]。花生青枯病防治难度大,种植抗病品种是最经济有效的措施。自上世纪七十年代以来,相继建立了田间自然病圃法[2-3]、浸种法[4]、针刺法[5]、剪叶法[6]等花生青枯病抗性鉴定技术。In order to improve the efficiency and reliability of bacterial wilt resistance screening in peanut,a novel water culture inoculation system was developed.Resistant peanut variety‘Zhonghua21’and susceptible‘Zhonghua12’were inoculated with mixed suspension of three Ralstonia solanacearum isolates under(28±2)℃,and 16 h light/8 h dark conditions.The minimum concentration for distinguishing the resistant and suscepti-ble peanut lines was 1×10^(7 )CFU·mL^(-1).The optimal time for scoring the resistance was 12 d after inoculation.36 peanut accessions were inoculated by the mixed bacterial suspension in water culture,in which 25 ones were re-sistant and 11 ones were susceptible.The same set of peanut accessions was also tested for their resistance to bac-terial wilt in natural disease nursery,from which 27 ones were resistant and nine were susceptible.The consisten-cy of these accessions for resistance to bacterial wilt both in water culture and in natural disease nursery was more than 80%.Therefore,the established method was quick,simple and high throughput for identifying resist-ance to bacterial wilt in peanut.

关 键 词：花生青枯病 种植抗病品种 危害面积 防治难度 青枯病抗性 病圃 剪叶 青枯菌 

分 类 号：S432.44[农业科学—植物病理学]