柳树丛枝病病原分子鉴定  

Molecular identification of the pathogen of willow witches’-broom

作　　者：陈雅寒 武崇高 方健惠 朱亚灵 许宏刚 程晓月 欧阳经政 CHEN Yahan;WU Chonggao;FANG Jianhui;ZHU Yaling;XU Honggang;CHENG Xiaoyue;OUYANG Jingzheng(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China;Forestry pest control and quarantine station of Gansu,Lanzhou 730070,China;Institute of Landscape Science,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070 [2]甘肃省林业有害生物防治检疫站,兰州7300703 [3]兰州市园林科学研究所,兰州730070

出　　处：《植物病理学报》2024年第1期220-224,共5页Acta Phytopathologica Sinica

基　　金：甘肃农业大学人才启动金项目(GAU-KYQD-2019-22);林业科技创新与国际合作省级财政资金项目([2019]418);兰州市科技局项目(2022-2-49);国家自然科学基金(32160628);甘肃省自然科学基金(21JR7RA820);甘肃省高等学校创新基金项目(2021B-117)。

摘　　要：柳树(Willow)是杨柳科(Salicaceae)柳属(Salix)乔木植物,大多数品种具有耐旱耐盐碱的特性,对环境具有良好的适应能力[1]。柳树在甘肃省内广泛种植,是风景林、防护林和水土保持林的重要组成树种。柳树丛枝病是一种典型的植原体病害,当寄主植株被病原物侵染时,顶芽生长受到抑制而侧芽提前萌发,导致受害枝条无法正常抽条或木质化,严重时甚至引起整株枯死[2],对林业的生态、经济和社会效益造成了极大损失。近年来,柳树丛枝病在甘肃省各市州发生严重,已经引起省内各界的普遍关注。To clarify the taxonomic status of the phytoplasma causing willow witches’-broom in Gansu Pro-vince,this study used nested PCR technique to clone the 16S rDNA gene using total DNA collected from differ-ent regions and species of willow witches’-broom samples as templates,and nine 1250 bp target fragments were obtained using P1/P7 and R16F2n/R16R2;the sequences of the above genes were compared by homolo-gy,The results showed that the 16S rDNA gene of willow witches’-broom phytoplasma strain had the highest homology with Willow proliferation phytoplasma(Willow proliferation)in Inner Mongolia,both above 99.1%;the phylogenetic tree was constructed and found that the 16S rDNA gene of willow witches’-broom clustered with members of 16SrVI-A subgroup.The phylogenetic tree was constructed,and the 16S rDNA gene of willow witches’-broom clustered with members of the 16SrVI-A subgroup;and the virtual RFLP analysis reported that the highest sequence similarity with the one obtained in this study was the reference pattern of the 16SrVI-A subgroup,with a similarity coefficient around 0.98,so the willow witches’-broom phytoplasma in Gansu Province could be classified into the 16SrVI-A subgroup.Meanwhile,the phytoplasma resources were preserved by grafting and identified as vector insects based on amplification of the 16S rDNA gene in the willow shoulder reticulata.

关 键 词：丛枝病 水土保持林 风景林 抽条 柳树 分子鉴定 防护林 乔木植物 

分 类 号：S432.44[农业科学—植物病理学]