秦岭滑蜥断尾再生伤口愈合阶段细胞因子的时空分布特征  

The Spatial-Temporal Distribution of Cytokines in the Wound Healing Stage of Regenerating Tail in Scincella tsinlingensis

作　　者：杨纯[1] 师婷婷 王新月 寇曌婷 孙金玉 YANG Chun;SHI Ting-Ting;WANG Xin-Yue;KOU Zhao-Ting;SUN Jin-Yu(School of Life Sciences,Shanxi Normal University,Taiyuan 030031,China)

机构地区：[1]山西师范大学生命科学学院,太原030031

出　　处：《动物学杂志》2024年第2期245-257,共13页Chinese Journal of Zoology

基　　金：山西省基础研究计划自由探索类项目(No.20210302123323);山西师范大学自然科学基金项目(No.020820220007)。

摘　　要：细胞因子参与免疫应答、介导炎症反应,进而在损伤组织的再生修复过程中发挥调控作用。通过切割法建立秦岭滑蜥(Scincella tsinlingensis)断尾再生模型,针对伤口愈合阶段,采用免疫组织化学方法检测促炎症因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-1β(IL-1β)、白细胞介素-1受体1(IL-1R1)、肿瘤坏死因子-α(TNF-α)和抗炎细胞因子白细胞介素-10(IL-10)的组织细胞定位及表达变化。结果显示:6种细胞因子在断尾脊髓和椎骨中的骨系细胞中均呈阳性。断尾创面组织及其附近鳞片下方真皮层中IL-6阳性细胞数量先增加后减少,断尾后3 d达峰值194.3±24.9。断尾后3 d,创面IL-8阳性细胞数目为107.7±12.7,超过真皮层的43.7±9.1。IL-1β和IL-1R1阳性细胞数量先增后减,伤口部位的阳性细胞明显多于真皮层(P<0.05)。TNF-α阳性细胞由0.5 d的126.3±35.0增至3 d的190.3±12.1。IL-10阳性细胞断尾后总体呈现先增后减的趋势,断尾后0.5 d伤口部位阳性细胞达201±17.8,显著高于原尾(P<0.05)。上述结果表明,促炎症因子IL-6、IL-8、IL-1β及其受体IL-1R1、TNF-α和抗炎细胞因子IL-10与创面髓系来源细胞的空间分布相一致,共同参与秦岭滑蜥断尾伤口愈合,并可能通过维持免疫抑制的微环境实现无疤痕伤口愈合,以促进芽基形成。[Objectives]Cytokines participate in the immune response,mediate the inflammatory response,and then play a regulatory role in the regeneration and repair process of damaged tissues.[Methods]The tail of Scincella tsinlingensis was amputated with a razor blade to set up tail regeneration model,and immunohistochemical methods were used to detect the histologic localization and expression changes of cytokines interleukin,IL-6,8,1β(IL-6,IL-8,IL-1β),interleukin 1 receptor type 1(IL-1R1),tumour necrosis factor-α(TNF-α)and interleukin,IL-10(IL-10)at the stage of wound healing.Three visual fields of different parts were randomly selected under microscope with 400-fold magnification for immunopositive cell count.The results were expressed as“Mean±SD”,one-way ANOVA and Tukey HSD were used to analyze the difference in the number of positive cells on different days.[Results]The results showed that six cytokines were positive in bone cells in the spinal cord and vertebrae.The number of IL-6 positive cells in the wound site and the dermis of proximal scale to the stump surface initially showed increasing and later decreasing of these cells,reaching a peak of 194.3±24.9 at 3 d after tail amputation,the number of IL-8 positive cells in the wound surface was 107.7±12.7,exceeding 43.7±9.1 in the dermis at 3 d after tail amputation.The number of IL-1βand IL-1R1 positive cells initially showed increasing and later decreasing of these cells,and significantly more positive cells occurred in the wound site than in the dermis(P<0.05).The TNF-αpositive cells increased from 126.3±35.0 at 0.5 d to 190.3±12.1 at 3 d.The number of anti-inflammatory cytokine IL-10 positive cells initially showed increasing and later decreasing of these cells after tail amputation.At 0.5 d after tail amputation,positive cells reached 201±17.8,which is significantly higher than that in the original tail(P<0.05,Fig.7).[Conclusion]The above results indicate that IL-6,IL-8,IL-1β,IL-1R1,TNF-αand IL-10 are consistent with the spatial distribution o

关 键 词：秦岭滑蜥 断尾再生 伤口愈合 细胞因子 

分 类 号：Q954[生物学—动物学]