白塞病患者外周血T淋巴细胞亚群及细胞因子与其临床特征关系分析  

Analysis of peripheral blood T lymphocyte subsets and cytokines and their correlation with clinical manifestations in Behcet's disease patients

作　　者：毛玉景[1] 焦占峰[2] 程盼盼[3] 宋芹[1] 郭向华[1] 李健[1] 张丽丽[1] 蒋雨平 邵莉[1] 闵亚楠 MAO Yujing;JIAO Zhanfeng;CHENG Panpan;SONG Qin;GUO Xianghua;LI Jian;ZHANG Lili;JIANG Yuping;SHAO Li;MIN Yanan(Department of Rheumatology,Affiliated Hospital of Jining Medical University,Jining 272029,China;Department of Nephrology,Affiliated Hospital of Jining Medical University,Jining 272029,China;Department of Laboratory,Affiliated Hospital of Jining Medical University,Jining 272029,China)

机构地区：[1]济宁医学院附属医院风湿免疫科,济宁272029 [2]济宁医学院附属医院肾内科,济宁272029 [3]济宁医学院附属医院实验室,济宁272029

出　　处：《国际免疫学杂志》2024年第1期39-45,共7页International Journal of Immunology

基　　金：国家自然科学基金(81700110);山东省中医药科技发展计划项目(2019-0492);济宁市重点研发计划项目(2021YXNS014)。

摘　　要：目的检测白塞病(Behcet’s disease,BD)患者外周血T细胞亚群及细胞因子水平,并分析BD患者不同临床特征对外周血T细胞亚群及细胞因子水平的影响。方法选取2018年1月至2021年12月在济宁医学院附属医院诊治的BD患者80例作为病例组,另选取本院健康体检中心40例健康志愿者作为对照组。采用流式细胞术检测外周血辅助性T细胞(helper T cell,Th)1、Th17、调节性T细胞(regulatory T cell,Treg)亚群表达。酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法测定血清细胞因子干扰素-γ(interferon-γ,IFN-γ)、白细胞介素(interleukin,IL)-4、IL-10、IL-17、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平。结果与对照组相比,BD患者总T淋巴亚群、CD8+T及Th1细胞表达水平均显著升高,差异有统计学意义{%:[68.75(54.23,80.32)]比[72.10(62.23,86.45)],[23.44(12.89,33.76)]比[31.46(20.13,45.26)],[10.67(8.23,12.35)]比[17.96(10.26,25.39)],Z=-3.13、-4.54、-3.97,P值均<0.05},而Th、Th17及Treg细胞表达的组间差异无统计学意义(P>0.05)。与对照组相比,BD患者IL-17、IL-6、TNF-α、IL-4、IFN-γ血清水平均显著升高,血清IL-10水平显著降低,差异均有统计学意义(Z=-4.32、-5.01、-8.18、-8.70、-3.48、-8.30,P值均<0.05)。与无相关表现患者相比,有关节炎表现的BD患者和活动性BD患者IL-6表达显著升高,差异有统计学意义{pg/mL:[3.23(2.98,5.35)]比[10.51(6.72,23.21)],[6.32(4.79,8.93)]比[9.68(6.97,18.73)],Z=5.47、8.76,P值均<0.05}。与无相关临床表现患者相比有关节炎表现、眼部表现、消化道表现和活动性BD患者TNF-α水平显著升高,同时有消化系统病变的BD患者IFN-γ水平显著升高,差异均有统计学意义{pg/mL:[7.74(6.89,10.19)]比[39.84(30.37,50.61)],[6.12(5.36,9.89)]比[31.35(15.71,30.46)],[6.49(4.78,10.21)]比[19.89(14.36,36.21)],[5.89(4.61,8.96)]比[27.91(15.32,37.81)],[6.89(5.43,14.86)]比[26.79(15.41,31.56)],Z=7.70、6.84、6.94、9.47、5.70,Objective To detect peripheral blood T lymphocyte subsets and cytokines in Behcet's disease(BD)patients,and analyze the impact of different clinical characteristics on peripheral blood T cell subpopulations and cytokine levels in BD patients.Methods Eighty patients with BD who were diagnosed and treated at the Affiliated Hospital of Jining Medical University from January 2018 to December 2021 were selected as the case group.Another forty healthy volunteers from the health examination center of our hospital were selected as control group.Helper T cell(Th)1,Th17,and regulatory T cell(Treg)lymphocyte subsets were detected by flow cytometry.Enzyme linked immunosorbent assay(ELISA)was used to detect the serum levels of interferon-γ(IFN-γ),interleukin(IL)-4,IL-10,IL-17,IL-6,tumor necrosis factor-α(TNF-α).Results Compared with the control group,the expression levels of total T lymphocyte subpopulations,CD8+T and Th1 cell in BD patients were significantly increased{%:[68.75(54.23,80.32)]vs[72.10(62.23,86.45)],[23.44(12.89,33.76)]vs[31.46(20.13,45.26)],[10.67(8.23,12.35)]vs[17.96(10.26,25.39)],Z=-3.13,-4.54,-3.97,all P values<0.05},while there was no statistically significant difference in the expression of Th,Th17 and Treg cells between the groups(P>0.05).Compared with the control group,the serum levels of IFN-γ、IL-4、IL-17、IL-6 and TNF-α were increased and IL-10 was decreased significantly in the BD group(Z=-4.32,-5.01,-8.18,-8.70,-3.48,-8.30,all P values<0.05).Compared to patients without related manifestations,the expression levels of IL-6 in patients with osteoarthritis and active BD were significantly increased{pg/mL:[3.23(2.98,5.35)]vs[10.51(6.72,23.21)],[6.32(4.79,8.93)]vs[9.68(6.97,18.73)],Z=5.473,8.763,both P values<0.05}.Compared to patients without relevant clinical manifestations,the expression levels of TNF-α were significantly increased in patients with arthritis,ocular manifestations,gastrointestinal manifestations and active BD,and the IFN-γwas significantly increased in BD patients with dige

关 键 词：白塞病 临床特征 T细胞亚群 细胞因子 

分 类 号：R597[医药卫生—内科学]