Spearman与GRA法联合熵值法解析双黄连口服液解热抗炎药效物质基础  

Spearman and GRA method combined with entropy method to analyze the material basis of antipyretic and anti-inflammatory effects of Shuanghuanglian Oral Liquid

作　　者：高燕[1] 丁晓彦[2] 刘璐[1] 朱日然[3] 赵渤年 GAO Yan;DING Xiaoyan;LIU Lu;ZHU Riran;ZHAO Bonian(Institute of Pharmaceutical Research,Shandong University of Traditional Chinese Medicine,High Level Traditional Chinese Medicine Key Disciplines of National Administration of Traditional Chinese Medicine,Pharmaceutics of Traditional Chinese Medicine,Collaborative Innovation Center of Ecological Protection and High Quality Development of Characteristic Traditional Chinese Medicine in the Yellow River Basin,Jinan 250355,China;Shandong Academy of Traditional Chinese Medicine,Jinan 250014,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学药物研究院,国家中医药管理局高水平重点学科-中药药剂学,黄河流域特色中药生态保护和高质量发展协同创新中心,济南250355 [2]山东省中医药研究院,济南250014 [3]山东中医药大学附属医院,济南250014

出　　处：《中华中医药杂志》2024年第4期1985-2000,共16页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省重点研发计划(No.2017CXGC1306,No.2021CXGC010511)。

摘　　要：目的:研究双黄连口服液解热抗炎的药效物质基础。方法:体内药效实验考察双黄连口服液对脂多糖炎症致热大鼠体内炎症因子的抑制作用,采集49批双黄连口服液样本的UPLC-Q-Exactive Orbitrap-MS指纹图谱和综合药效数据,利用Spearman秩相关性分析和灰色关联度综合分析双黄连口服液解热抗炎药效物质群。结果:建立了双黄连口服液原型成分指纹图谱检测方法,对69种化学成分进行了定量表征;体内实验显示不同厂家的双黄连口服液对大鼠肛温和白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α均有抑制作用;通过Spearman秩相关性分析和灰色关联度联合分析,解析了双黄连口服液中29个解热抗炎有效成分。结论:本研究初步揭示了双黄连口服液发挥解热抗炎作用的关键质量标志物,为双黄连口服液的二次开发及质量评价提供了技术支撑。Objective:To study the material basis of antipyretic and anti-inflammatory effects of Shuanghuanglian Oral Liquid.Methods:The inhibitory effect of Shuanghuanglian Oral Liquid on inflammatory cytokines in rats induced by lipopolysaccharide inflammation was investigated by pharmacodynamic experiment in vivo.UPLC-Q-Exactive-Orbitrap-MS fingerprint quantitative data and comprehensive pharmacodynamic data of 49 batches of Shuanghuanglian Oral Liquid samples were collected.Spearman rank correlation coefficient analysis(SCC)and gray relational analysis(GRA)were used to analyze the antipyretic and anti-inflammatory substances of Shuanghuanglian Oral Liquid comprehensively.Results:The UPLC-Q-Exactive-Orbitrap-MS method was established for the detect prototype components of Shuanghuanglian Oral Liquid,and 69 chemical components were quantitatively characterized.In vivo pharmacodynamic experiments showed that Shuanghuanglian Oral Liquid from different manufacturers could inhibit IL-6,IL-1βand TNF-αin different degrees.The comprehensive efficacy index value was calculated by the entropy method.According to the characteristics of sample data,29 antipyretic and anti-infammatory active components in Shuanghuanglian Oral Liquid were analyzed by SCC and GRA methods.Conclusion:The study preliminically reveales the key quality marker of Shuanghuanglian Oral Liquid for its antifebriosis and anti-inflammatory effects,which provides technical support for the secondary development and quality evaluation of Shuanghuanglian Oral Liquid.

关 键 词：双黄连口服液 药效物质 斯皮尔曼秩相关性分析 灰色关联度分析 熵值法 

分 类 号：R285.5[医药卫生—中药学]