藏药普尔那黄酮纯化工艺及抗氧化活性研究  

Purification technology and antioxidant activity of Tibetan medicine Artemisia vestita Wall.ex Bess flavonoids

作　　者：邱钺姿 徐凤华 陈静 夏青 张云 刘可春 林厚文[5] 张姗姗 李晓彬 QIU Yuezi;XU Fenghua;CHEN Jing;XIA Qing;ZHANG Yun;LIU Kechun;LIN Houwen;ZHANG Shanshan;LI Xiaobin(Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China;Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province,Jinan 250103,China;School of Basic Medicine,Qingdao University,Qingdao 266000,China;University of Tibetan Medicine,Lhasa 850000,China;Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)

机构地区：[1]齐鲁工业大学(山东省科学院),山东省科学院生物研究所,济南250103 [2]山东省人类疾病斑马鱼模型与药物筛选工程技术研究中心,济南250103 [3]青岛大学基础医学院,青岛266000 [4]西藏藏医药大学,拉萨850000 [5]上海交通大学医学院附属仁济医院,上海200127

出　　处：《中华中医药杂志》2024年第4期2010-2015,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.42006090);西藏藏医药大学2020年中医学(藏医)博士点建设项目(No.BSDJS-20-04,No.BSDJS-20-18);山东省重点研发计划(重大科技创新工程)(No.2021CXGC010507);济南市高校20条资助项目(No.2020GXRC031);藏医药区域协同创新中心项目(No.2019XTCX011);泰山学者特聘专家项目(No.ts20190950)。

摘　　要：目的:以藏药普尔那为原料,开展其黄酮纯化工艺及抗氧化活性研究。方法:采用静态吸附与解吸试验,考察8种不同型号大孔树脂对普尔那黄酮吸附-解吸性能,筛选出最佳大孔树脂型号,通过对工艺各参数优化,确定黄酮最佳纯化工艺,以体外自由基清除实验、还原力实验和基于斑马鱼模型的体内抗氧化活性为考察指标,比较纯化前后普尔那黄酮抗氧化能力的差异。结果:普尔那黄酮最佳纯化工艺为:采用DA-201-CIV型大孔树脂,在上样液浓度为1.2 mg/mL,上样速度为2 BV/h,上样体积为3 BV,洗脱溶剂为70%乙醇溶液,洗脱速度为2 BV/h的条件下,收集0.7~2 BV洗脱范围内洗脱液,可使普尔那黄酮含量达到(38.60±0.57)%。抗氧化试验结果表明,普尔那黄酮具有较强的清除自由基能力以及良好的还原力,纯化后的普尔那黄酮在一定浓度范围内可抑制由甲硝唑诱导的斑马鱼皮肤荧光细胞氧化损伤。结论:通过大孔树脂纯化,可使普尔那黄酮纯度提升1.84倍,基于体外抗氧化试验及斑马鱼模型,明确了普尔那黄酮的抗氧化能力,其可用于抗氧化剂产品的开发。Objective:To study the purification process and antioxidant activity of flavonoids from Tibetan medicine Artemisia vestita Wall.ex Bess.Methods:Static adsorption test was used to investigate the adsorption and desorption performance of 8 different types of macroporous resin for flavonoids of Artemisia vestita Wall ex Bess,and the optimal macroporous resin model was selected.The optimal purification process of flavonoids was determined by optimizing the process parameters.In vitro free radical scavenging assay,reducing power assay and antioxidant activity in vivo based on zebrafish model were used to compare the difference of antioxidant capacity of Artemisia vestita Wall.ex Bess flavonoids before and after purification.Results:The best purification process of Artemisia vestita Wall.ex Bess flavonoids was:DA-201-CIV macroporous resin was used to collect the eluent in the range of 0.7~2 BV under the conditions of loading solution concentration of 1.2 mg/mL,loading speed of 2 BV/h,loading volume of 3 BV,eluting solvent of 70%ethanol,and elution speed of 2 BV/h.The content of Artemisia vestita Wall.ex Bess flavonoids could reach(38.60+0.57)%.Antioxidant test results showed that Artemisia vestita Wall.ex Bess flavonoids had strong scavenging ability and good reducing power,and purified Artemisia vestita Wall.ex Bess flavonoids could inhibit the oxidative damage of skin fluorescence cells induced by metronidazole in a certain concentration range.Conclusion:Purified by macroporous resin,the purity of Artemisia vestita Wall.ex Bess flavonoids is increased by 1.84 times.Based on in vitro antioxidant test and zebrafish model,the antioxidant capacity of Artemisia vestita Wall.ex Bess fiavonoids is determined,which could be used in the development of antioxidant products.

关 键 词：藏药普尔那 黄酮 大孔树脂 斑马鱼 抗氧化活性 

分 类 号：R29[医药卫生—民族医学]