Genome-wide analysis of Panonychus citrimicro RNAs witha focus on potential insecticidal activity of 4 micro RNAs toeggs and nymphs  

作　　者：Chuan-Zhen Li Yu-Hang Liu Deng Pan Meng-Hao Xia Qiang Zhang Yu-Chuang Li Guo-Rui Yuan Jin-Jun Wang Wei Dou 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Academy of Agricultural Sciences,SouthwestUniversity,Chongqing,China [2]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),Academy of Agricultural Sciences,Southwest University,Chongqing,China

出　　处：《Insect Science》2024年第2期354-370,共17页昆虫科学（英文版）

基　　金：supported by the National Natural Sci-ence Foundation of China(31972272,31871969);the Fundamental Research Funds for the Central Universi-ties(SWU-XDPY22001)of China;the China Agri-cultural Research System of MOA and MARA。

摘　　要：Panonychus citriMcGregor(Acari:Tetranychidae),a destructive citrus pest,causes considerable annual economic losses due to its short lifespan and rapid resistancedevelopment.MicroRNA(miRNA)-induced RNA interference is a promising approachfor pest control because of endogenous regulation of pest growth and development.Tosearch for miRNAs with potential insecticidal activity inP.citri,genome-wide analysis ofmiRNAs at different developmental stages was conducted,resulting in the identificationof 136 miRNAs,including 73 known and 63 novel miRNAs.A total of 17 isomiRNAsand 12 duplicated miRNAs were characterized.MiR-1 and miR-252-5p were identified asreference miRNAs forP.citriandTetranychus urticae.Based on differential expressionanalysis,treatments with miR-let-7a and miR-315 mimics and the miR-let-7a antagomirsignificantly reduced the egg hatch rate and resulted in abnormal egg development.Over-expression or downregulation of miR-34-5p and miR-305-5p through feeding significantlydecreased the adult eclosion rate and caused molting defects.The 4 miRNAs,miR-let-7a,miR-315,miR-34-5p,and miR-305-5p,had important regulatory functions and insectici-dal properties in egg hatching and adult eclosion.In general,these data advance our un-derstanding of miRNAs in mite biology,which can assist future studies on insect-specificmiRNA-based green pest control technology.

关 键 词：growth and development MICRORNAS Panonychus citri referencemiRNAs REGULATION small RNA libraries 

分 类 号：Q96[生物学—昆虫学]