Acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica  

作　　者：Xiaojin Pei Tiantian Bai Yuan Luo Zhanfeng Zhang Sheng Li Yongliang Fan Tong-Xian Liu 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology and Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau,Ministry of Agriculture,Northwest A&F University,Yangling,Shaanxi,China [3]Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou,Guangdong Province,China [4]Institute of Entomology,Guizhou University,Guiyang,China

出　　处：《Insect Science》2024年第2期387-404,共18页昆虫科学（英文版）

基　　金：funded by the National Natural Science Foundation of China(32200384);the China Postdoctoral Science Foundation(2022M710053).

摘　　要：Lipid and sugar homeostasis is critical for insect development and survival.In this study,we characterized an acetyl coenzyme A carboxylase gene in Blattella germanica(BgACC)that is involved in both lipogenesis and sugar homeostasis.We found that BgACC was dominantly expressed in the fat body and integument,and was significantly upregulated after molting.Knockdown of BgACC in 5th-instar nymphs did not affect their normal molting to the next nymphal stage,but it caused a lethal phenotype during adult emergence.BgACC-RNA interference(RNAi)significantly downregulated total free fatty acid(FFA)and triacylglycerol(TAG)levels,and also caused a significant decrease of cuticular hydrocarbons(CHCs).Repression of BgACC in adult females affected the development of oocytes and resulted in sterile females,but BgACC-RNAi did not affect the reproductive ability of males.Interestingly,knockdown of BgACC also changed the expression of insulin-like peptide genes(BglLPs),which mimicked a physiological state of high sugar uptake.In addition,BgACC was upregulated when B.germanica were fed on a high sucrose diet,and repression of BgACC upregulated the expression of the glycogen synthase gene(BgGlyS).Moreover,BgACC-RNAi increased the circulating sugar levels and glycogen storage,and a longevity assay suggested that BgACC was important for the survival of B.germanica under conditions of high sucrose uptake.Our results confirm that BgACC is involved in multiple lipid biogenesis and sugar homeostasis processes,which further modulates insect reproduction and sugar tolerance.This study benefits our understanding of the crosstalk between lipid and sugar metabolism.

关 键 词：acetyl-CoA carboxylase German cockroach lipid biosynthesis sugar tolerance 

分 类 号：Q96[生物学—昆虫学]