Identification of an odorant receptor responding to sex pheromones in Spodoptera frugiperda extends the novel type-I PR lineage in moths  

作　　者：Sai Zhang Emmanuelle Jacquin-Joly Nicolas Montagne Fang Liu Yang Liu Guirong Wang 

机构地区：[1]College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Bejing,China [3]Institute of Ecology and En vironmental Sciences of Paris,INRAE,Sorbonne University,CNRS,IRD,UPEC,University of Paris,Versailles,France [4]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China

出　　处：《Insect Science》2024年第2期489-502,共14页昆虫科学（英文版）

基　　金：supported by the National Key R&D Program of China(2022YFD1400800 and 2022YFE0116500);Central Public-interest Scientific Institution Basal Research Fund(CAASZDRW202108);the Agricultural Science and Technology Innovation Program.Research was conducted as part of the CAAS-INRAE Associated International Laboratory on Plant Protection,with the support of the French Embassy in China(COMIX 2019-2020).

摘　　要：moths,pheromone receptors(PRs)are crucial for intraspecific sexual communication between males and females.Moth PRs are considered as an ideal model for studying the evolution of insect PRs,and a large number of PRs have been identified and functionally characterized in different moth species.Moth PRs were initially thought to fall into a single monophyletic clade in the odorant receptor(OR)family,but recent studies have shown that ORs in another lineage also bind type-I sex pheromones,which indicates that type-I PRs have multiple independent origins in the Lepidoptera.In this study,we investigated whether ORs of the pest moth Spodoptera frugiperda belonging to clades closely related to this novel PR lineage may also have the capacity to bind type-I pheromones and serve as male PRs.Among the 7 ORs tested,only 1(SfruOR23)exhibited a male-biased expression pattern.Importantly,in vitro functional characterization showed that SfruOR23 could bind several type-I sex pheromone compounds with Z-9-tetradecenal(Z9-14:Ald),a minor component found in female sex pheromone glands,as the optimal ligand.In addition,SfruOR23 also showed weak responses to plant volatile organic compounds.Altogether,we characterized an S.frugiperda PR positioned in a lineage closely related to the novel PR clade,indicating that the type-I PR lineage can be extended in moths.

关 键 词：odorant receptor pheromone receptor lineage sex pheromone Spodoptera frugiperda 

分 类 号：Q96[生物学—昆虫学]