Both host and diet shape bacterial communities of predatory mites  

作　　者：Hong Yan Endong Wang Guo-Shu Wei Bo Zhang Xuenong Xu 

机构地区：[1]Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Bejing,China [2]College of Plant Protection,Hebei Agricultural University,Baoding,Hebei Province,China [3]Key Laboratory of Natural Enemies Insects,Ministry of Agriculture and Rural Affairs,Beijing,China

出　　处：《Insect Science》2024年第2期551-561,共11页昆虫科学（英文版）

基　　金：supported by National Natural Science Foundation of China(Grant No.32070402);Beijing Natural Science Foundation(Grant No.6222052);Beijing Agriculture Innovation Consortium(BAIC01-2022);Agricultural Science and Technology Innovation Program,CAAS“Protection Application of Insect Natural Enemies".

摘　　要：Microbial communities,derived from food,ambient,and inner,can affect host ecological adaption and evolution.Comparing with most phytophagous arthropods,predators may have more opportunities to develop specific microbiota depending on the level of prey specialization.To explore how diet sources affect host microbial communities and vary across predator species,we considered 3 types of predators from Phytoseiidae(Acari:Mesostigmata):polyphagous(Amblyseius orientalis Ehara,Neoseiulus barkeri Hughes,and Amblyseius swirski Athias-Henrio),oligophagous(Neoseiulus californicus McGregor),and monophagous(Phytoseiulus persimilis Athias-Henriot)predatory mites.The polyphagous species were fed on 2 types of diets,natural prey and alternative prey.By using 16S rRNA sequencing,we found that diet was the main source of microbiota in predatory mites,while there was no clear pattern affected by prey specialization.Among 3 polyphagous predators,host species had a larger impact than prey on microbial composition.Unlike A.orientalis or N.barkeri which showed consistency in their microbiota,prey switching significantly affectedβ-diversity of bacterial composition in A.swirskii,with 56%of the microbial alteration.In short,our results confirmed the substantial influence of diet on host microbial construction in predatory species,and highlighted species differences in shaping the microbiota which are not necessarily related to prey specialization.

关 键 词：diet switching feeding habits host-microbial interactions invertebrate microbiota 16S rRNA 

分 类 号：Q96[生物学—昆虫学]