Mitogenome-based phylogeny of mosquitoes(Diptera:Culicidae)  

作　　者：De-Hong Chen Shu-Lin He Wen-Bo Fu Zhen-Tian Yan Yun-Jian Hu Huan Yuan Ming-Bin Wang Bin Chen 

机构地区：[1]Chongqing Key Laboratory of Vector Insects,Institute of Entomology and Molecular Biology,Chongqing Normal University,Chongqing,China

出　　处：《Insect Science》2024年第2期599-612,共14页昆虫科学（英文版）

基　　金：supported by the following:The National Natural Science Foundation of China(31872262,31672363);National Key Program of Science and Technology Foundation Work of China(2015FY210300);Science and Technology Research Program of Chongqing Education Commission of China(KJQN202000504,KJQN202200566).

摘　　要：Mosquitoes are of great medical significance as vectors of many deadly diseases.Mitogenomes have been widely used in phylogenetic studies,but mitogenome knowledge within the family Culicidae is limited,and Culicidae phylogeny is far from resolved.In this study,we surveyed the mitogenomes of 149 Culicidae species,including 7 newly sequenced species.Comparative analysis of 149 mosquito mitogenomes shows gene composition and order to be identical to that of an ancestral insect,and the AT bias,length variation,and codon usage are all consistent with that of other reported Dipteran mitogenomes.Phylogenetic analyses based on the DNA sequences of the 13 protein-coding genes from the 149 species robustly support the monophyly of the subfamily Anophelinae and the tribes Aedini,Culicini,Mansonini,Sabethini,and Toxorhynchitini.To resolve ambiguous relationships between clades within the subfamily Culicinae,we performed topological tests and show that Aedini is a sister to Culicini and that Uranotaenini is a sister to(Mansonini)+(Toxorhynchitini+Sabethini).In addition,we estimated divergence times using a Bayesian relaxation clock based on the sequence data and 3 fossil calibration points.The results show mosquitoes diverged during the Early Jurassic with massive Culicinae radiations during the Cretaceous,coincident with the emergence of angiosperms and the burst of mammals and birds.Overall,this study,which uses the largest number of Culicidae mitogenomes sequenced to date,comprehensively reveals the mitogenome characteristics and mitogenome-based phylogeny and divergence times of Culicidae,providing information for further studies on the mitogenome,phylogeny,evolution,and taxonomic revision of Culicidae.

关 键 词：characterization CULICIDAE EVOLUTION mitogenomes MOSQUITOES PHYLOGENY 

分 类 号：Q95[生物学—动物学]